Moderate dependence of reactive oxygen species production on membrane potential in avian muscle mitochondria oxidizing glycerol 3-phosphate

Motoi Kikusato, Masaaki Toyomizu

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    Mitochondria are a major source of reactive oxygen species production in cells, and the production level is sensitive to the magnitude of the membrane potential (ΔΨ). The present study investigated the level of superoxide production in mitochondria oxidizing glycerol 3-phosphate (GP) and its dependence on ΔΨ in isolated avian muscle mitochondria. The levels of superoxide produced in mitochondria oxidizing GP were lower than those obtained with succinate and were similar to those obtained with NADH-linked substrates (glutamate/malate/pyruvate). The dependence of superoxide production on ΔΨ in mitochondria oxidizing GP was lower than that of mitochondria oxidizing succinate, and a weak dependence of GP-supported superoxide production on ΔΨ was observed in the presence of NADH-linked substrates or succinate. These results suggest that the levels of superoxide generated in response to GP are quantitatively low, but they are unsusceptible to changes in ΔΨ in avian muscle mitochondria.

    Original languageEnglish
    Pages (from-to)555-559
    Number of pages5
    JournalJournal of Physiological Sciences
    Volume65
    Issue number6
    DOIs
    Publication statusPublished - 2015 Nov 1

    Keywords

    • Carbonyl cyanide p-trifluoromethoxyphenyl hydrazine
    • Mitochondrial glycerol 3-phosphate dehydrogenase
    • Mitochondrial membrane potential

    ASJC Scopus subject areas

    • Physiology

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