Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast

Takashi Itoh, Akio Toh-E, Yasushi Matsui

Research output: Contribution to journalArticlepeer-review

87 Citations (Scopus)

Abstract

Class V myosins play a pivotal role in organelle distribution. In the budding yeast, Myo2p, a class V myosin, is essential for mitochondrial distribution. We identified MMR1 as a high-dose suppressor of the myo2 mitochondrial defect and that Mmr1p resides restrictively on the bud-localizing mitochondria and forms a complex with Myo2p tail. Mmr1p loss delayed mitochondrial transfer to buds and completely abolished mitochondrial distribution in the absence of Ypt11p, which promotes mitochondrial distribution by complex formation with Myo2p tail. The myo2-573 mutation, which causes a mitochondrial distribution defect and inactivates the Mmr1p function, reduced association between Myo2p and Mmr1p and depolarized Mmr1p localization on mitochondria. These strongly suggest that Mmr1p is a key mitochondrial component of the link between Myo2p and mitochondria for Myo2p-dependent mitochondrial distribution. Genetical analysis revealed that the Mmr1p-Myo2p pathway is independent of the Ypt11p-Myo2p pathway, suggesting that an essential system for mitochondrial distribution is composed of two independent Myo2p pathways.

Original languageEnglish
Pages (from-to)2520-2530
Number of pages11
JournalEMBO Journal
Volume23
Issue number13
DOIs
Publication statusPublished - 2004 Jul 7
Externally publishedYes

Keywords

  • Mitochondria
  • Myosin
  • Organeile distribution
  • Yeast

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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