MIZ1, an essential protein for root hydrotropism, is associated with the cytoplasmic face of the endoplasmic reticulum membrane in Arabidopsis root cells

Tomokazu Yamazaki, Yutaka Miyazawa, Akie Kobayashi, Teppei Moriwaki, Nobuharu Fujii, Hideyuki Takahashi

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    MIZ1 is encoded by a gene essential for root hydrotropism in Arabidopsis. To characterize the property of MIZ1, we used transgenic plants expressing GFP-tagged MIZ1 (MIZ1-GFP) and mutant MIZ1 (MIZ1G235E-GFP) in a miz1-1 mutant. Although both chimeric genes were transcribed, the translational products of MIZ1G235E-GFP did not accumulate in roots. Moreover, MIZ1-GFP complemented the mutant phenotype but not MIZ1G235E-GFP. The signal corresponding to MIZ1-GFP was detected at high levels in cortical cells and lateral root cap cells and accumulated in compartments in cortical cells. MIZ1-GFP was fractionated into a soluble protein fraction and an endoplasmic reticulum (ER) membrane fraction, where it was bound to the surface of the ER membrane at the cytosolic side.

    Original languageEnglish
    Pages (from-to)398-402
    Number of pages5
    JournalFEBS Letters
    Volume586
    Issue number4
    DOIs
    Publication statusPublished - 2012 Feb 17

    Keywords

    • DUF617
    • Endoplasmic reticulum
    • MIZ1
    • Plant root hydrotropism
    • Protein localization

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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