Mitogenic Activity of Cytoplasmic Membranes Isolated from L-Forms of Staphylococcus aureus

Haruhiko Takada, Yoshiyuki Hirachi, Hideo Hashizume, Shozo Kotani

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Cytoplasmic membranes of L-forms of Staphylococcus aureus exerted a strong mitogenic effect on splenocytes of athymic nude mice as well as normal mice, while a cytoplasmic fraction of the same bacteria did not show definite mitogenicity. The mitogenic principle(s) of the membrane fraction was resistant to treatment with trypsin and was heat stable (at 100 C for 10 min). The active principle (s) in the insoluble residue of the membrane fraction digested with trypsin was not extracted with cold acetone, but could be solubilized by extraction with a cold chloroform-methanol mixture (2:1, v/v). The mitogenic principle(s) in the extract was fractionated by silicic acid column chromatography. Among five fractions separated by chromatography, fractions eluted with chloroform-methanol mixtures (1:1 and 1:20, v/v) were found to be strongly mitogenic. The cytoplasmic membranes of the L-forms also exerted a definite mitogenic effect on guinea pig splenocytes, but not on the thymocytes.

Original languageEnglish
Pages (from-to)1079-1090
Number of pages12
JournalMICROBIOLOGY and IMMUNOLOGY
Volume24
Issue number11
DOIs
Publication statusPublished - 1980 Jan 1

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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