Mitochondrial DNA repair by photolyase

Akira Yasui, Hirohiko Yajima, Takayasu Kobayashi, Andre P.M. Eker, Atsushi Oikawa

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Photolyase genes of Saccharomyces cerevisiae and Escherichia coli were expressed in S. cerevisiae and photoreactivation in nuclei and mitochondria of the host cells was analyzed by determination of survival and petit rates. Yeast photolyase was able to repair mitochondrial DNA effectively, whereas E. coli photolyase could reduce only a small fraction of the petit rate produced by UV irradiation. Analysis using fusion between yeast photolyase and E. coli lacZ genes as well as a chimeric gene between yeast and E. coli photolyase genes suggests the importance of the protruding amino terminal region of the yeast photolyase for its transport into mitochondria. A significant similarity between the protruding amino termini of yeast photolyase and yeast uracil-DNA-glycosylase suggests a common functional importance of the terminal sequences for both DNA repair enzymes.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalMutation Research-DNA Repair
Volume273
Issue number2
DOIs
Publication statusPublished - 1992 Mar

Keywords

  • Mitochondrial DNA
  • Photoreactivation
  • Protein transport
  • Uracil-DNA-glycosylase
  • Yeast

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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