Mitf-D, a newly identified isoform, expressed in the retinal pigment epithelium and monocyte-lineage cells affected by Mitf mutations

Kazuhisa Takeda, Ken Ichi Yasumoto, Naoko Kawaguchi, Tetsuo Udono, Ken Ichi Watanabe, Hideo Saito, Kazuhiro Takahashi, Masaki Noda, Shigeki Shibahara

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

Microphthalmia-associated transcription factor (Mitf) regulates the differentiation of melanocytes, optic cup-derived retinal pigment epithelium (RPE), and some types of bone marrow-derived cells. Mitf consists of at least five isoforms with different N-termini, each of which is encoded by a separate exon 1. Here we identified a novel isoform, termed mouse Mitf-D/human MITF-D, that is expressed in RPE, macrophages, and osteoclasts affected by the Mitf mutations, but not expressed in other Mitf target cells, including melanocyte-lineage cells and natural killer cells. The initiation Met of MITF-D is located in the downstream domain (B1b domain) that is shared by other MITF isoforms. The 5′-untranslated region of MITF-D mRNA is encoded by the newly identified first exon of the MITF gene, termed exon 1D, which is located 3 kb upstream of the exon encoding the B1b domain. Thus, the MITF gene generates multiple isoforms with different expression patterns by using the alternative promoters in a cell-dependent manner, thereby providing the molecular basis for the phenotypic variability seen in the MITF/Mitf mutants.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1574
Issue number1
DOIs
Publication statusPublished - 2002 Feb 20

Keywords

  • Cell differentiation
  • Macrophage
  • Mitf
  • Osteoclast
  • Retinal pigment epithelium
  • Transcription

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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