miranda localizes staufen and prospero asymmetrically in mitotic neuroblasts and epithelial cells in early Drosophila embryogenesis

Fumio Matsuzaki, Tomokazu Ohshiro, Hiroko Ikeshima-Kataoka, Hitomi Izumi

Research output: Contribution to journalArticlepeer-review

97 Citations (Scopus)

Abstract

When neuroblasts divide, prospero protein and mRNA segregate asymmetrically into the daughter neuroblast and sibling ganglion mother cell. miranda is known to localize prospero protein to the basal cell cortex of neuroblasts while the staufen RNA-binding protein mediates prospero mRNA localization. Here we show that miranda is required for asymmetric staufen localization in neuroblasts. Analyses using miranda mutants reveal that prospero and staufen interact with miranda under the same cell-cycle-dependent control. miranda thus acts to partition both prospero protein and mRNA. Furthermore, miranda localizes prospero and staufen to the basolateral cortex in dividing epithelial cells, which express the three proteins prior to neurogenesis. Our observations suggest that the epithelial cell and neuroblast (both of epithelial origin) share the same molecular machinery for creating cellular asymmetry.

Original languageEnglish
Pages (from-to)4089-4098
Number of pages10
JournalDevelopment
Volume125
Issue number20
Publication statusPublished - 1998 Nov 23
Externally publishedYes

Keywords

  • Asymmetric division
  • Drosophila
  • Epithelial polarity
  • Miranda
  • Neuroblast
  • Prospero
  • Staufen

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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