MiR-210 regulates the interaction between pancreatic cancer cells and stellate cells

Tetsuya Takikawa, Atsushi Masamune, Shin Hamada, Eriko Nakano, Naoki Yoshida, Tooru Shimosegawa

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

There is accumulating evidence that pancreatic stellate cells (PSCs) promote the progression of pancreatic cancer. microRNAs (miRNAs) are small non-coding RNAs acting as negative regulators of gene expression at the post-transcriptional level. This study aimed to clarify the role of miRNAs in the interaction between PSCs and pancreatic cancer cells. Pancreatic cancer cells were mono-cultured or indirectly co-cultured with PSCs. miRNAs were prepared, and Agilent's miRNA microarray containing probes for 904 human miRNAs was used to identify differentially expressed miRNAs. miR-210 was identified as an upregulated miRNA by co-culture with PSCs. Conditioned media of PSCs activated ERK and Akt, but not hypoxia-inducible factor-1α pathway. PSCs-induced miR-210 upregulation was inhibited by inhibitors of ERK and PI3K/Akt pathways. Inhibition of miR-210 expression decreased migration, decreased the expression of vimentin and snai-1, and increased the membrane-associated expression of β-catenin in Panc-1 cells co-cultured with PSCs. In conclusion, our results suggest a novel role of miR-210 in the interaction between PSCs and pancreatic cancer cells.

Original languageEnglish
Pages (from-to)433-439
Number of pages7
JournalBiochemical and biophysical research communications
Volume437
Issue number3
DOIs
Publication statusPublished - 2013 Aug 2

Keywords

  • Fibrosis
  • MicroRNA
  • Pancreatic cancer
  • Pancreatitis
  • Stroma

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'MiR-210 regulates the interaction between pancreatic cancer cells and stellate cells'. Together they form a unique fingerprint.

Cite this