Miniaturized thermal lens and fluorescence detection system for microchemical chips

Masayo Yamauchi, Manabu Tokeshi, Jun Yamaguchi, Takashi Fukuzawa, Akihiko Hattori, Akihide Hibara, Takehiko Kitamori

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


We have developed a miniaturized two-way detection system using thermal lens and fluorescence spectroscopies for microchip chemistry. The system was composed of laser diode (LD) modules, fiber-based optics combined with a gradient index lens, and miniaturized detection units for thermal lens and fluorescence signals. The detection limits in the thermal lens and fluorescence spectroscopies were 6.3 × 10-9 M for Ni(II) phthalocyanine tetrasulfonic acid and 3.0 × 10-9 M for cy5, respectively. The performance of the system with the miniaturized thermal lens was equivalent to that of a conventional thermal lens microscope. The fluorescence sensitivity was comparable to sensitivities offered by conventional miniaturized systems.

Original languageEnglish
Pages (from-to)89-93
Number of pages5
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - 2006 Feb 17
Externally publishedYes


  • Fluorescence detection
  • Microchannel
  • Microchip
  • Thermal lens detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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