Mineral trioxide aggregate inhibits osteoclastic bone resorption

D. Hashiguchi, H. Fukushima, H. Yasuda, W. Masuda, M. Tomikawa, K. Morikawa, K. Maki, E. Jimi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Mineral trioxide aggregate (MTA), a commonly used endodontic repair material, is useful for both basic and clinical research, and the effect of MTA on osteoblast differentiation has been well-defined. However, the effects of MTA on osteoclastic bone resorption are not fully understood. Hence, the aim of this study is to examine the effect of MTA solution in the regulation of osteoclast bone-resorbing activity using osteoclasts formed in co-cultures of primary osteoblasts and bone marrow cells. MTA solution dosedependently reduced the total area of pits formed by osteoclasts. The reduction of resorption induced by 20% MTA treatment was due to inhibition of osteoclastic bone-resorbing activity and had no effect on osteoclast number. A 20% MTA solution disrupted actin ring formation, a marker of osteoclastic bone resorption, by reducing phosphorylation and kinase activity of c-Src, and mRNA expressions of cathepsin K and mmp-9. A high concentration of MTA solution (50%) induced apoptosis of osteoclasts by increasing the expression of Bim, a member of the BH3-only (Bcl-2 homology) family of pro-apoptotic proteins. Taken together, our results suggest that MTA is a useful retrofilling material for several clinical situations because it both stimulates osteoblast differentiation and inhibits bone resorption.

Original languageEnglish
Pages (from-to)912-917
Number of pages6
JournalJournal of dental research
Volume90
Issue number7
DOIs
Publication statusPublished - 2011 Jul 1

Keywords

  • Apoptosis
  • Bone resorption
  • Mineral trioxide aggregate
  • Osteoclast
  • RANKL
  • Src

ASJC Scopus subject areas

  • Dentistry(all)

Fingerprint Dive into the research topics of 'Mineral trioxide aggregate inhibits osteoclastic bone resorption'. Together they form a unique fingerprint.

  • Cite this

    Hashiguchi, D., Fukushima, H., Yasuda, H., Masuda, W., Tomikawa, M., Morikawa, K., Maki, K., & Jimi, E. (2011). Mineral trioxide aggregate inhibits osteoclastic bone resorption. Journal of dental research, 90(7), 912-917. https://doi.org/10.1177/0022034511407335