Migration, integration, and differentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord

Sufan Wu, Yoshihisa Suzuki, Masaaki Kitada, Miyako Kitaura, Kazuya Kataoka, Jun Takahashi, Chizuka Ide, Yoshihiko Nishimura

Research output: Contribution to journalArticlepeer-review

93 Citations (Scopus)

Abstract

Hippocampus-derived neurospheres were prepared from transgenic rat fetuses expressing green fluorescent protein (GFP), and transplanted into an alginate-filled lesion of young rat spinal cord. One, two and four weeks after transplantation, a large number of grafted cells survived, many of which expressed immunoreactivity for glial fibrillary acidic protein, and a few expressed immunoreactivity for β-tubulin III. The grafted cells closely attached to the host tissue including astrocytes at the border of the lesion. It was notable that numerous GFP-positive cells had migrated within host spinal cord tissue up to 2 mm away from the implanted site 4 weeks postoperation. These results demonstrate that rat fetal hippocampus-derived neurosphere cells could survive, differentiate, extensively migrate, and integrate well into the host spinal cord tissue.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalNeuroscience Letters
Volume312
Issue number3
DOIs
Publication statusPublished - 2001 Oct 26

Keywords

  • Alginate
  • Differentiation
  • Green fluorescent protein-transgenic rat
  • Hippocampus
  • Migration
  • Neural stem cell
  • Neurosphere
  • Spinal cord regeneration

ASJC Scopus subject areas

  • Neuroscience(all)

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