Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence

Hiroyuki Oikawa, Yuta Suzuki, Masataka Saito, Kiyoto Kamagata, Munehito Arai, Satoshi Takahashi

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protein A labeled with donor and acceptor fluorophores suggest conformational heterogeneity and dynamic fluctuations in the unfolded state.

Original languageEnglish
Article number2151
JournalScientific reports
Volume3
DOIs
Publication statusPublished - 2013

ASJC Scopus subject areas

  • General

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