Five novel microsatellite loci were isolated from Pacific abalone Haliotis discus discus, and the polymorphisms were examined to estimate genetic variability. The genetic variabilities varied depending on the locus: the number of alleles ranged from 3 to 10, and the observed and expected hetrozygosity ranged from 0.17 to 0.80, and 0.20 to 0.89, respectively. Two loci showed significant Hardy-Weinberg disequilibrium at the 0.01 < P < 0.05 levels. The high variabilities revealed in this study were evidently higher than that observed in allozyme loci, suggesting that these microsatellites should prove useful for various genetic investigations.
- Haliotis discus discus
- Microsatellite DNA
- Pacific abalone
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)