MicroRNAs in plasma and cerebrospinal fluid as potential markers for Alzheimer's disease

Takehiro Kiko, Kiyotaka Nakagawa, Tsuyoshi Tsuduki, Katsutoshi Furukawa, Hiroyuki Arai, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

138 Citations (Scopus)

Abstract

The development of Alzheimer's disease (AD) biomarkers remains an unmet challenge, and new approaches that can improve current AD biomarker strategies are needed. Recent reports suggested that microRNA (miRNA) profiling of biological fluids has emerged as a diagnostic tool for several pathologic conditions. In this study, we measured six candidate miRNAs (miR-9, miR-29a, miR-29b, miR-34a, miR-125b, and miR-146a) in plasma and cerebrospinal fluid (CSF) of AD and normal subjects by using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) to evaluate their potential usability as AD biomarkers. The qRT-PCR results showed that plasma miR-34a and miR-146a levels, and CSF miR-34a, miR-125b, and miR-146a levels in AD patients were significantly lower than in control subjects. On the other hand, CSF miR-29a and miR-29b levels were significantly higher than in control subjects. Our results provide a possibility that miRNAs detected in plasma and CSF can serve as biomarkers for AD.

Original languageEnglish
Pages (from-to)253-259
Number of pages7
JournalJournal of Alzheimer's Disease
Volume39
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • Alzheimer's disease
  • cerebrospinal fluid
  • miRNA
  • plasma

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

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