MicroRNA conjugated gold nanoparticles and cell transfection

Elizabeth Crew, Sharaara Rahman, Asma Razzak-Jaffar, Derrick Mott, Martha Kamundi, Gang Yu, Nuri Tchah, Jehwan Lee, Michael Bellavia, Chaun Jian Zhong

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

While the importance of microRNAs (miRNAs) in cancer treatment or manipulation of genetic expression has been increasingly recognized for developing miRNA-based therapies, the controlled delivery of miRNAs into specific cells constitutes a challenging task. This report describes preliminary findings from an investigation of the conjugation of gold nanoparticles with miRNAs (miRNA-AuNPs) and their cell transfection. The immobilization of miRNAs on the AuNPs was detected, and the surface stability was substantiated by gel electrophoresis assessment of the highly charged characteristics of miRNA-AuNPs and their surface-exchange inactivity with a highly charged surfactant. The miRNA-AuNPs were tested in cell transfection using multiple myeloma cells, demonstrating efficient knockdown in the functional luciferase assay. The findings have important implications for understanding the mechanistic details of cell transfection involving miRNA-conjugated nanoparticles as biosensing or targeting probes.

Original languageEnglish
Pages (from-to)26-29
Number of pages4
JournalAnalytical Chemistry
Volume84
Issue number1
DOIs
Publication statusPublished - 2012 Jan 3
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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