MicroRNA-34b functions as a potential tumor suppressor in endometrial serous adenocarcinoma

Eri Hiroki, Fumihiko Suzuki, Jun Ichi Akahira, Satoru Nagase, Kiyoshi Ito, Jun Ichi Sugawara, Yasuhiro Miki, Takashi Suzuki, Hironobu Sasano, Nobuo Yaegashi

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Endometrial serous adenocarcinoma (ESC) is aggressive and carries a poor prognosis. p53 is frequently mutated in ESC. microRNAs (miRNAs) are a direct p53 target and have been implicated in cancer cell behavior. In this study, we compared miRNA expression levels in ESC with the levels in endometrial endometrioid adenocarcinoma (EEC) and normal endometria. Six miRNAs were identified as having aberrant down-regulation specific to ESC with miR-34b being most pronounced. miR-34b was found to have promoter hypermethylation, which when reversed, restored miR-34b expression in the cell lines treated with 5-aza-2' deoxycytidine (DAC). Ectopic expression of miR-34b in turn inhibited cell growth, migration and most notably invasion. Our findings suggest a relationship among p53 mutation, miR-34b promoter methylation and tumor cell behavior. These effects are likely mediated by the downstream target of miR-34b, the proto-oncogene mesenchymal-epithelial transition factor (MET), a known prognostic factor in endometrial carcinomas. The expression of MET was reduced following the restoration of miR-34b in cell lines. In summary, our data suggest that miR-34b plays a role in the molecular pathogenesis of endometrial cancer.

Original languageEnglish
Pages (from-to)E395-E404
JournalInternational Journal of Cancer
Volume131
Issue number4
DOIs
Publication statusPublished - 2012 Aug 15

Keywords

  • MET
  • endometrial cancer
  • miR-34b
  • microRNA
  • serous

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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