Abstract
We developed a novel technique to observe individual cells in various environments using a cage made of patterned hydrogel on polydimethylsiloxane (PDMS) microfluidic chips. The hydrogel is polymerized by UV-ray illumination (300-400 nm) on a glass surface, which is spin coated with the water-soluble prepolymer solution, and immobilized on the glass surface. The hydrogel is hydrophilic, transparent, biocompatible. Then, the PDMS microchannel is assembled and bonded on it. We transported cells into a cage using optical tweezers. In the cage, solution flow is stable and chemicals permeate through the wall, so cells are not flowed and we can change chemical conditions around cells stably. We succeeded in making a system to observe cells stably for long time.
Original language | English |
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Pages | 1861-1863 |
Number of pages | 3 |
Publication status | Published - 2008 Jan 1 |
Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16 |
Other
Other | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
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Country/Territory | United States |
City | San Diego, CA |
Period | 08/10/12 → 08/10/16 |
Keywords
- Cage
- Cell handling & analysis
- Hydrogel
- Optical tweezers
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering