Micropatterning of hydrogel and on-chip long time monitoring of individual cells in a cage

Fumihito Arai, Hideyuki Matsumoto, Toshiaki Shijuku, Nobuyuki Uozumi

Research output: Contribution to conferencePaperpeer-review

Abstract

We developed a novel technique to observe individual cells in various environments using a cage made of patterned hydrogel on polydimethylsiloxane (PDMS) microfluidic chips. The hydrogel is polymerized by UV-ray illumination (300-400 nm) on a glass surface, which is spin coated with the water-soluble prepolymer solution, and immobilized on the glass surface. The hydrogel is hydrophilic, transparent, biocompatible. Then, the PDMS microchannel is assembled and bonded on it. We transported cells into a cage using optical tweezers. In the cage, solution flow is stable and chemicals permeate through the wall, so cells are not flowed and we can change chemical conditions around cells stably. We succeeded in making a system to observe cells stably for long time.

Original languageEnglish
Pages1861-1863
Number of pages3
Publication statusPublished - 2008 Jan 1
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Keywords

  • Cage
  • Cell handling & analysis
  • Hydrogel
  • Optical tweezers

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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