Micropatterning of hydrogel and on-chip long time monitoring of individual cells in a cage

Fumihito Arai; Hideyuki Matsumoto; Toshiaki Shijuku; Nobuyuki Uozumi

Micropatterning of hydrogel and on-chip long time monitoring of individual cells in a cage

Fumihito Arai, Hideyuki Matsumoto, Toshiaki Shijuku, Nobuyuki Uozumi

ENG - Biomolecular Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

We developed a novel technique to observe individual cells in various environments using a cage made of patterned hydrogel on polydimethylsiloxane (PDMS) microfluidic chips. The hydrogel is polymerized by UV-ray illumination (300-400 nm) on a glass surface, which is spin coated with the water-soluble prepolymer solution, and immobilized on the glass surface. The hydrogel is hydrophilic, transparent, biocompatible. Then, the PDMS microchannel is assembled and bonded on it. We transported cells into a cage using optical tweezers. In the cage, solution flow is stable and chemicals permeate through the wall, so cells are not flowed and we can change chemical conditions around cells stably. We succeeded in making a system to observe cells stably for long time.

Original language	English
Pages	1861-1863
Number of pages	3
Publication status	Published - 2008 Jan 1
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country	United States
City	San Diego, CA
Period	08/10/12 → 08/10/16

Keywords

Cage
Cell handling & analysis
Hydrogel
Optical tweezers

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

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Micropatterning of hydrogel and on-chip long time monitoring of individual cells in a cage. / Arai, Fumihito; Matsumoto, Hideyuki; Shijuku, Toshiaki; Uozumi, Nobuyuki.

2008. 1861-1863 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

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AU - Shijuku, Toshiaki

AU - Uozumi, Nobuyuki

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N2 - We developed a novel technique to observe individual cells in various environments using a cage made of patterned hydrogel on polydimethylsiloxane (PDMS) microfluidic chips. The hydrogel is polymerized by UV-ray illumination (300-400 nm) on a glass surface, which is spin coated with the water-soluble prepolymer solution, and immobilized on the glass surface. The hydrogel is hydrophilic, transparent, biocompatible. Then, the PDMS microchannel is assembled and bonded on it. We transported cells into a cage using optical tweezers. In the cage, solution flow is stable and chemicals permeate through the wall, so cells are not flowed and we can change chemical conditions around cells stably. We succeeded in making a system to observe cells stably for long time.

AB - We developed a novel technique to observe individual cells in various environments using a cage made of patterned hydrogel on polydimethylsiloxane (PDMS) microfluidic chips. The hydrogel is polymerized by UV-ray illumination (300-400 nm) on a glass surface, which is spin coated with the water-soluble prepolymer solution, and immobilized on the glass surface. The hydrogel is hydrophilic, transparent, biocompatible. Then, the PDMS microchannel is assembled and bonded on it. We transported cells into a cage using optical tweezers. In the cage, solution flow is stable and chemicals permeate through the wall, so cells are not flowed and we can change chemical conditions around cells stably. We succeeded in making a system to observe cells stably for long time.

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KW - Cell handling & analysis

KW - Hydrogel

KW - Optical tweezers

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