TY - GEN
T1 - Micromesh culture technique enabling cell maturation for the use of organ on a chip
AU - Kurosawa, Osamu
AU - Hori, Takeshi
AU - Tani, Nobutaka
AU - Iwata, Hiroo
N1 - Funding Information:
We would like to thank the valuable discussions and suggestions of Professor Washizu of the University of Tokyo and Professor Okeyo of Kyoto University. This research was supported by grants from JSPS KAKENHI No. 18K12071, and the “Compass to Healthy Life” Research Complex Program of JST.
Funding Information:
We would like to thank the valuable discussions and suggestions of Professor Washizu of the University of Tokyo and Professor Okeyo of Kyoto University. This research was supported by grants from JSPS KAKENHI No. 18K12071, and the ?Compass to Healthy Life? Research Complex Program of JST.
Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Recently, we have developed a novel cell culture method named “micromesh cell culture technique”. By using this technique, hiPSCs could be differentiated into trophectoderm in base culture medium for iPS cells without any additional supplements. In this study, we used this technique at the aim of realizing maturation of organ cells to be used for "organ on a chip". In the result of HepG2, an increase in gene expression of albumin and CYP1A2 which is one of drug metabolizing enzyme was confirmed by mesh culture. In addition, By co-culturing with fibroblast on micromesh, Caco-2 showed the increase in expression of tight junction-related gene and CYP1A2.
AB - Recently, we have developed a novel cell culture method named “micromesh cell culture technique”. By using this technique, hiPSCs could be differentiated into trophectoderm in base culture medium for iPS cells without any additional supplements. In this study, we used this technique at the aim of realizing maturation of organ cells to be used for "organ on a chip". In the result of HepG2, an increase in gene expression of albumin and CYP1A2 which is one of drug metabolizing enzyme was confirmed by mesh culture. In addition, By co-culturing with fibroblast on micromesh, Caco-2 showed the increase in expression of tight junction-related gene and CYP1A2.
KW - Cell maturation
KW - HiPSC
KW - Micromesh
KW - Organ on a chip
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M3 - Conference contribution
AN - SCOPUS:85079826653
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1638
EP - 1640
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -