Micromesh culture technique enabling cell maturation for the use of organ on a chip

Osamu Kurosawa, Takeshi Hori, Nobutaka Tani, Hiroo Iwata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recently, we have developed a novel cell culture method named “micromesh cell culture technique”. By using this technique, hiPSCs could be differentiated into trophectoderm in base culture medium for iPS cells without any additional supplements. In this study, we used this technique at the aim of realizing maturation of organ cells to be used for "organ on a chip". In the result of HepG2, an increase in gene expression of albumin and CYP1A2 which is one of drug metabolizing enzyme was confirmed by mesh culture. In addition, By co-culturing with fibroblast on micromesh, Caco-2 showed the increase in expression of tight junction-related gene and CYP1A2.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1638-1640
Number of pages3
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Externally publishedYes
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume3

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period18/11/1118/11/15

Keywords

  • Cell maturation
  • HiPSC
  • Micromesh
  • Organ on a chip

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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