Microinjection of exogenous DNA into eggs of halocynthia roretzi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Exogenous gene expression assays during development, including reporters under the control of 5′ upstream enhancer regions of genes, constitute a powerful technique for understanding the mechanisms of tissue-specific gene expression regulation and determining the characteristics, behaviors, and functions of cells that express these genes. The simple marine chordate Halocynthia roretzi has been used for these transgenic analyses for a long time and is an excellent model system for such studies, especially in comparative analyses with other ascidians. In this study, I describe simple methods for microinjecting H. roretzi eggs with exogenous DNA, such as a promoter construct consisting of a 5′ upstream region and a reporter gene, which are prerequisites for transgenic analyses. I also describe basic knowledge regarding this ascidian species, providing reasons why it is an ideal subject for developmental biology studies.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages25-35
Number of pages11
DOIs
Publication statusPublished - 2018 Jan 1

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1029
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Enhancer regions
  • Fertilized eggs
  • Halocynthia roretzi
  • Microinjection
  • Micromanipulator
  • Ooplasmic segregation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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