In this paper, we proposed a microfluidic chip having a world-to-chip interface for multi-scale environmental measurement on a chip. There are several techniques for environmental measurement of the microscale objects using cantilever, microsensor and fluorescence method etc. Each technique has useful advantages against other methods. To employ these methods on a microfluidic chip simultaneously, we aim to develop a world-to-chip interface to insert a cantilever into the microfluidic chip and position it for sensing in a chip. We fabricated the microfluidic chip having this interface to insert the cantilever. This chip was designed to be used for inverted microscope. The solution does not leak from the interface by employing simultaneous flow control at both inlet and drain port. By using this interface, we demonstrated insertion and positioning of the cantilever into the microfluidic chip, and confirmed two-layer laminar flow control and detection of the local temperature change in the microchannel.