TY - JOUR
T1 - Microfabrication and Characterization of Solid Surfaces Patterned with Enzymes or Antigen-Antibodies by Scanning Electrochemical Microscopy
AU - Shiku, H.
AU - Hara, Y.
AU - Takeda, T.
AU - Matsue, T.
AU - Uchida, I.
PY - 1997
Y1 - 1997
N2 - The microfabrication and characterization of glass surfaces patterned with enzymes (diaphorase, horseradish peroxidase(HRP)) or antigen-antibodies (carcinoembryonic antigen (CEA), human chorionic gonadotropin (HCG) and human placental lactogen (HPL)) were studied using scanning electrochemical microscopy (SECM). Localized enzymes and antigen-antibody complexes with labeled enzymes were characterized on the basis of detection of catalytic current for ferrocenylmethanol by SECM. The SECM technique was extended to the enzyme-linked immunosorbent assay (ELISA). This method detects as low as ∼ 104 CEA molecules in a single microspot. We also demonstrated a novel dual assay using microfabricated glass substrates with anti-HCG and anti-HPL microspots.
AB - The microfabrication and characterization of glass surfaces patterned with enzymes (diaphorase, horseradish peroxidase(HRP)) or antigen-antibodies (carcinoembryonic antigen (CEA), human chorionic gonadotropin (HCG) and human placental lactogen (HPL)) were studied using scanning electrochemical microscopy (SECM). Localized enzymes and antigen-antibody complexes with labeled enzymes were characterized on the basis of detection of catalytic current for ferrocenylmethanol by SECM. The SECM technique was extended to the enzyme-linked immunosorbent assay (ELISA). This method detects as low as ∼ 104 CEA molecules in a single microspot. We also demonstrated a novel dual assay using microfabricated glass substrates with anti-HCG and anti-HPL microspots.
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U2 - 10.1021/bk-1997-0656.ch015
DO - 10.1021/bk-1997-0656.ch015
M3 - Article
AN - SCOPUS:0346510793
VL - 656
SP - 202
EP - 209
JO - ACS Symposium Series
JF - ACS Symposium Series
SN - 0097-6156
ER -