Microfabrication and Characterization of Solid Surfaces Patterned with Enzymes or Antigen-Antibodies by Scanning Electrochemical Microscopy

H. Shiku, Y. Hara, T. Takeda, T. Matsue, I. Uchida

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The microfabrication and characterization of glass surfaces patterned with enzymes (diaphorase, horseradish peroxidase(HRP)) or antigen-antibodies (carcinoembryonic antigen (CEA), human chorionic gonadotropin (HCG) and human placental lactogen (HPL)) were studied using scanning electrochemical microscopy (SECM). Localized enzymes and antigen-antibody complexes with labeled enzymes were characterized on the basis of detection of catalytic current for ferrocenylmethanol by SECM. The SECM technique was extended to the enzyme-linked immunosorbent assay (ELISA). This method detects as low as ∼ 104 CEA molecules in a single microspot. We also demonstrated a novel dual assay using microfabricated glass substrates with anti-HCG and anti-HPL microspots.

Original languageEnglish
Pages (from-to)202-209
Number of pages8
JournalACS Symposium Series
Volume656
DOIs
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Fingerprint Dive into the research topics of 'Microfabrication and Characterization of Solid Surfaces Patterned with Enzymes or Antigen-Antibodies by Scanning Electrochemical Microscopy'. Together they form a unique fingerprint.

Cite this