Microfabrication and Characterization of Diaphorase-Patterned Surfaces by Scanning Electrochemical Microscopy

Hitoshi Shiku, Toshihiro Takeda, Hiroshi Yamada, Tomokazu Matsue, Isamu Uchida

Research output: Contribution to journalArticlepeer-review

125 Citations (Scopus)

Abstract

Scanning electrochemical microscopy (SECM) was used to microfabricate and quantify diaphorase-pattemed glass surfaces. Deactivated circular and linear micropattems were produced at diaphorase-immobilized substrates by a localized surface reaction. The oxidation of Br- and Cl- at a microelectrode generated a reactive species which deactivated the localized enzyme molecules at the substrate. The diaphorase-patterned surfaces were characterized by SECM on the basis of detection of catalytic current of ferrocenylmethanol coupled with oxidation of NADH. The concentration of the immobilized diaphorase was mapped from the quantitative analysis of the catalytic current.

Original languageEnglish
Pages (from-to)312-317
Number of pages6
JournalAnalytical Chemistry
Volume67
Issue number2
DOIs
Publication statusPublished - 1995 Jan 1

ASJC Scopus subject areas

  • Analytical Chemistry

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