Microelastic mapping of living endothelial cells exposed to shear stress in relation to three-dimensional distribution of actin filaments

Masaaki Sato, Kenichi Suzuki, Yosuke Ueki, Toshiro Ohashi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The surface topography and local elastic moduli of endothelial cells exposed to shear stress were measured using atomic force microscopy. Bovine aortic endothelial cells were exposed to shear stress of 2 Pa for 6, 12 or 24 h. In addition, a confocal laser-scanning microscope used in conjunction with the atomic force microscope was used to observe the actin filament structure of these endothelial cells to elucidate the relationship between mechanical properties and cytoskeletal structure. The elastic modulus, calculated using the Hertz model, was measured at 50 × 50 points at 1 μm intervals within 40 min. For endothelial cells sheared for 6 h and 12 h, the elastic modulus at the upstream region was found to be higher than that at the downstream region. For endothelial cells sheared for 24 h, the elastic modulus at both the upstream and downstream regions increased. Fluorescent images showed thick, elongated actin filaments oriented in the direction of flow at the ventral surface of the cells. In the middle plane of the cells, actin filaments developed around the nucleus, while in the upper plane, short, thick actin filaments were observed but thick stress fibers were not present. The high elastic modulus came from the stress fibers. These results indicate that the higher elastic modulus observed in the upstream and downstream regions of sheared endothelial cells is mainly due to the development of stress fibers at the ventral surface and middle plane of the cell.

Original languageEnglish
Pages (from-to)311-319
Number of pages9
JournalActa Biomaterialia
Volume3
Issue number3 SPEC. ISS.
DOIs
Publication statusPublished - 2007 May

Keywords

  • Actin filament
  • Atomic force microscope
  • Elastic property
  • Endothelial cell
  • Shear stress

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Microelastic mapping of living endothelial cells exposed to shear stress in relation to three-dimensional distribution of actin filaments'. Together they form a unique fingerprint.

Cite this