Microdetermination of the 6,15-diketo-13,14-dihydro-prostaglandin F(1α) in human plasma using gas chromatography/selected ion monitoring with [18O]6,15-diketo-13,14-dihydro-prostaglandin F(1α) as an internal standard

Michinao Mizugaki, Takanori Hishinuma, Hiroaki Yamaguchi, Kazuhiko Kanno, Yuko Hirose, Hironori Nakamura

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We devised a simple and effective purification method for the microdetermination of 6,15-diketo-13,14-dihydro-prostaglandin F(1α) (DK), a metabolite of prostacyclin (PGI2). [18O]DK was synthesized from the repeated base-catalyzed hydrolysis of methyl ester derivatives in [18O] water to obtain an internal standard for the gas chromatography/selected ion monitoring (GC/SIM) of DK. The methyl ester-methoxime-tert-butyldimethylsilyl ether derivative was prepared, then gas chromatography/selected ion monitoring was carried out by monitoring the ion at m/z 613.4 for DK and at m/z 617.4 for an internal standard. A good linear response over the range of 10 pg to 10 ng was demonstrated. We detected DK to a level of about 297.8 pg/ml in human plasma. This method can be used to determine DK in biological samples.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalProstaglandins and Other Lipid Mediators
Volume58
Issue number2-4
DOIs
Publication statusPublished - 1999 Oct 1

Keywords

  • 6,15-diketo- 13,14-dihydro-prostaglandin F(1α)
  • Gas chromatography/mass spectrometry (GC/MS)
  • Human blood
  • Prostacyclin
  • Prostaglandin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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