Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography

Fumitaka Mafuné, Ken Miyajima, Kensuke Tono, Yoshihiro Takeda, Jun Ya Kohno, Naoya Miyauchi, Jun Kobayashi, Yasumasa Joti, Eriko Nango, So Iwata, Makina Yabashi

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 mm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 Å from <0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.

Original languageEnglish
Pages (from-to)520-523
Number of pages4
JournalActa Crystallographica Section D: Structural Biology
Volume72
Issue number4
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Liquid-droplet injector
  • Lysozyme
  • Serial femtosecond crystallography
  • X-ray free-electron laser

ASJC Scopus subject areas

  • Structural Biology

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