Microcontact printed diaphorase monolayer on glass characterized by atomic force microscopy and scanning electrochemical microscopy

Hong Qun Luo, Hitoshi Shiku, Ayako Kumagai, Yasufumi Takahashi, Tomoyuki Yasukawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Micropatterns of diaphorase (Dp) were fabricated on glass substrates by the microcontact printing (μCP) method and characterized with atomic force microscopy (AFM) and scanning electrochemical microscopy (SECM). AFM images of the printed samples revealed that the mean height of the Dp patterns was 3-5 nm, indicating the formation of a monolayer pattern. The Dp molecules on the surface organized themselves into two-dimensional arrays. We used two kinds of inking solutions: Dp-phosphate buffer solution (PBS) (pH 7.0) and Dp-PBS (pH 7.0) with glutaraldehyde (GA, 1% v/v) as a cross-linking reagent. Although the AFM imaging showed high-quality Dp monolayer patterns in both cases, SECM measurements indicated that the enzymatic activity of Dp was almost lost when Dp-PBS with GA was used as the inking solution, whereas clear enzymatic activity was found when Dp-PBS was used.

Original languageEnglish
Pages (from-to)2703-2708
Number of pages6
JournalElectrochemistry Communications
Volume9
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

Keywords

  • AFM
  • Diaphorase monolayer
  • Microcontact printing
  • SECM

ASJC Scopus subject areas

  • Electrochemistry

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