Microchip-based liquid-liquid extraction for gas-chromatography analysis of amphetamine-type stimulants in urine

Hajime Miyaguchi, Manabu Tokeshi, Yoshikuni Kikutani, Akihide Hibara, Hiroyuki Inoue, Takehiko Kitamori

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

A microchip-based liquid-liquid extraction for the gas chromatography analysis of urine for amphetamine-type stimulants has been developed. Partially modified microchannels with the capillarity restricted modification (CARM) method were employed for stabilizing the interface consisting of 1-chlorobutane and alkalized urine. Reliability of the microchip-based extraction was evaluated with respect to linearity, trueness and precision. As a practical demonstration, methoxyphenamine hydrochloride (50 mg) was administered to three healthy volunteers, and the concentration of methoxyphenamine in their urine was determined by both methods for comparison. This study showed the potential of pressure-driven microfluidics to contribute to the rapid automation analysis in forensic toxicology.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalJournal of Chromatography A
Volume1129
Issue number1
DOIs
Publication statusPublished - 2006 Sep 29
Externally publishedYes

Keywords

  • Amphetamines
  • Forensic toxicology
  • Gas chromatography
  • Microfluidics
  • Solvent extraction
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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