Microchanneled calorimetric concentration sensor for picoliter liquid samples of cytochrome c

Masaya Toda; Ning Xia; Naoki Inomata; Takahito Ono

doi:10.1541/ieejsmas.137.28

Microchanneled calorimetric concentration sensor for picoliter liquid samples of cytochrome c

Masaya Toda, Ning Xia, Naoki Inomata, Takahito Ono

ENG - Mechanical Systems Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

A calorimetric determination of protein concentration for a picoliter (pL) liquid sample is proposed using a microfluidic calorimeter on a thermally isolated membrane. The concentration measurement of cytochrome c in a pL solution based on an AC calorimeter by the absorbance of a 532 nm diode laser is demonstrated to monitor the temperature change of the microchannel. The resolution of the concentration is defined by measuring the temperature change against various concentrations. In the case of the fabricated micro-calorimeter, the heat resistance of the isolated membrane is obtained as 1579 K/W. The composed heat resistance, including the support membrane and the solution in the channel, is obtained as 118 K/W. Although the heat loss is extensive, the resolution is calculated as 3.3 mM as a concentration sensor with 1.5 mol/K sensitivity. The calorimetric device has more potential to be used for detection of extremely small concentrations in liquid by optimizing the thermal isolation with a higher concentration resolution.

Original language	English
Pages (from-to)	28-31
Number of pages	4
Journal	IEEJ Transactions on Sensors and Micromachines
Volume	137
Issue number	1
DOIs	https://doi.org/10.1541/ieejsmas.137.28
Publication status	Published - 2017

Keywords

Bio-sensing
Micro-calorimeter
Protein

ASJC Scopus subject areas

Mechanical Engineering
Electrical and Electronic Engineering

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10.1541/ieejsmas.137.28

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title = "Microchanneled calorimetric concentration sensor for picoliter liquid samples of cytochrome c",

abstract = "A calorimetric determination of protein concentration for a picoliter (pL) liquid sample is proposed using a microfluidic calorimeter on a thermally isolated membrane. The concentration measurement of cytochrome c in a pL solution based on an AC calorimeter by the absorbance of a 532 nm diode laser is demonstrated to monitor the temperature change of the microchannel. The resolution of the concentration is defined by measuring the temperature change against various concentrations. In the case of the fabricated micro-calorimeter, the heat resistance of the isolated membrane is obtained as 1579 K/W. The composed heat resistance, including the support membrane and the solution in the channel, is obtained as 118 K/W. Although the heat loss is extensive, the resolution is calculated as 3.3 mM as a concentration sensor with 1.5 mol/K sensitivity. The calorimetric device has more potential to be used for detection of extremely small concentrations in liquid by optimizing the thermal isolation with a higher concentration resolution.",

keywords = "Bio-sensing, Micro-calorimeter, Protein",

author = "Masaya Toda and Ning Xia and Naoki Inomata and Takahito Ono",

note = "Funding Information: Part of this work was performed at the Micro/Nanomachining Research and Education Center (MNC), Tohoku University. The authors gratefully acknowledge the support in part of the Special Coordination Funds for Promoting Science and Technology, Formation of Innovation Center for Fusion of Advanced Technologies from the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT), and the support in part by funds for Scientific Research from Japan Society for the Promotion of Science (JSPS). Publisher Copyright: {\textcopyright} 2017 The Institute of Electrical Engineers of Japan.",

year = "2017",

doi = "10.1541/ieejsmas.137.28",

language = "English",

volume = "137",

pages = "28--31",

journal = "IEEJ Transactions on Sensors and Micromachines",

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publisher = "The Institute of Electrical Engineers of Japan",

number = "1",

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TY - JOUR

T1 - Microchanneled calorimetric concentration sensor for picoliter liquid samples of cytochrome c

AU - Toda, Masaya

AU - Xia, Ning

AU - Inomata, Naoki

AU - Ono, Takahito

N1 - Funding Information: Part of this work was performed at the Micro/Nanomachining Research and Education Center (MNC), Tohoku University. The authors gratefully acknowledge the support in part of the Special Coordination Funds for Promoting Science and Technology, Formation of Innovation Center for Fusion of Advanced Technologies from the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT), and the support in part by funds for Scientific Research from Japan Society for the Promotion of Science (JSPS). Publisher Copyright: © 2017 The Institute of Electrical Engineers of Japan.

PY - 2017

Y1 - 2017

N2 - A calorimetric determination of protein concentration for a picoliter (pL) liquid sample is proposed using a microfluidic calorimeter on a thermally isolated membrane. The concentration measurement of cytochrome c in a pL solution based on an AC calorimeter by the absorbance of a 532 nm diode laser is demonstrated to monitor the temperature change of the microchannel. The resolution of the concentration is defined by measuring the temperature change against various concentrations. In the case of the fabricated micro-calorimeter, the heat resistance of the isolated membrane is obtained as 1579 K/W. The composed heat resistance, including the support membrane and the solution in the channel, is obtained as 118 K/W. Although the heat loss is extensive, the resolution is calculated as 3.3 mM as a concentration sensor with 1.5 mol/K sensitivity. The calorimetric device has more potential to be used for detection of extremely small concentrations in liquid by optimizing the thermal isolation with a higher concentration resolution.

AB - A calorimetric determination of protein concentration for a picoliter (pL) liquid sample is proposed using a microfluidic calorimeter on a thermally isolated membrane. The concentration measurement of cytochrome c in a pL solution based on an AC calorimeter by the absorbance of a 532 nm diode laser is demonstrated to monitor the temperature change of the microchannel. The resolution of the concentration is defined by measuring the temperature change against various concentrations. In the case of the fabricated micro-calorimeter, the heat resistance of the isolated membrane is obtained as 1579 K/W. The composed heat resistance, including the support membrane and the solution in the channel, is obtained as 118 K/W. Although the heat loss is extensive, the resolution is calculated as 3.3 mM as a concentration sensor with 1.5 mol/K sensitivity. The calorimetric device has more potential to be used for detection of extremely small concentrations in liquid by optimizing the thermal isolation with a higher concentration resolution.

KW - Bio-sensing

KW - Micro-calorimeter

KW - Protein

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Microchanneled calorimetric concentration sensor for picoliter liquid samples of cytochrome c

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Keywords

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