Microbiomechanical properties of cultured endothelial cells estimated by atomic force microscopy

M. Sato

Research output: Chapter in Book/Report/Conference proceedingChapter


Local mechanical properties were measured for bovine endothelial cells exposed to shear stress using an atomic force microscopy (AFM), and the AFM indentations were simulated by a finite element method (FEM) to determine the elastic modulus. After exposure to shear stress, the endothelial cells showed marked elongation and orientation in the flow direction, together with significant decrease in the peak cell height. The applied force-indentation depth curve was obtained at different locations of the cell surface and quantitatively expressed by the quadratic equation. The elastic modulus was determined by comparison of the experimental and numerical results. The modulus obtained in our FEM model significantly became higher from 12.2 ± 4.2 kPa to 18.7 ± 5.7 kPa with exposure to shear stress. Fluorescent images showed that stress fibers of F-actin bundles were mainly formed in the central portion of the sheared cells. The significant increase in the modulus may be due to the remodeling of cytoskeletal structure. The elastic modulus would contribute a better understanding of the mechanisms of endothelial cell remodeling processes during exposure to shear stress.

Original languageEnglish
Title of host publicationBiomechanics at Micro- and Nanoscale Levels
Subtitle of host publicationVolume I
PublisherWorld Scientific Publishing Co.
Number of pages10
ISBN (Electronic)9789812569301
ISBN (Print)981256098X, 9789812560988
Publication statusPublished - 2005 Jan 1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Immunology and Microbiology(all)
  • Medicine(all)


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