TY - JOUR
T1 - Microbiology
T2 - Direct imaging of intracellular signalling components that regulate bacterial chemotaxis
AU - Fukuoka, Hajime
AU - Sagawa, Takashi
AU - Inoue, Yuichi
AU - Takahashi, Hiroto
AU - Ishijima, Akihiko
PY - 2014/4/1
Y1 - 2014/4/1
N2 - The bacterial chemotaxis system regulates the rotational direction of flagellar motors through an intracellular signaling molecule, the phosphorylated form of CheY (CheY-P). The binding of CheY-P to a motor is believed to switch the motor's rotational direction from counterclockwise to clockwise. We demonstrated that the rotational switch of a motor was directly regulated by the binding and dissociation of CheY-P by simultaneously visualizing CheY tagged with green fluorescent protein and the rotational switching of a motor in live cells. The binding of 13 ± 7 CheY-P molecules was sufficient to induce clockwise rotation, and CheY-P molecules bound to and dissociated from a motor within ∼100 ms during switching. Thus, we have directly measured the regulation of the output from a signal transduction pathway by intracellular signaling proteins.
AB - The bacterial chemotaxis system regulates the rotational direction of flagellar motors through an intracellular signaling molecule, the phosphorylated form of CheY (CheY-P). The binding of CheY-P to a motor is believed to switch the motor's rotational direction from counterclockwise to clockwise. We demonstrated that the rotational switch of a motor was directly regulated by the binding and dissociation of CheY-P by simultaneously visualizing CheY tagged with green fluorescent protein and the rotational switching of a motor in live cells. The binding of 13 ± 7 CheY-P molecules was sufficient to induce clockwise rotation, and CheY-P molecules bound to and dissociated from a motor within ∼100 ms during switching. Thus, we have directly measured the regulation of the output from a signal transduction pathway by intracellular signaling proteins.
UR - http://www.scopus.com/inward/record.url?scp=84905640121&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84905640121&partnerID=8YFLogxK
U2 - 10.1126/scisignal.2004963
DO - 10.1126/scisignal.2004963
M3 - Article
C2 - 24692593
AN - SCOPUS:84905640121
VL - 7
SP - ra32
JO - Science's STKE : signal transduction knowledge environment
JF - Science's STKE : signal transduction knowledge environment
SN - 1937-9145
IS - 319
ER -