Microbiology: Direct imaging of intracellular signalling components that regulate bacterial chemotaxis

Hajime Fukuoka, Takashi Sagawa, Yuichi Inoue, Hiroto Takahashi, Akihiko Ishijima

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The bacterial chemotaxis system regulates the rotational direction of flagellar motors through an intracellular signaling molecule, the phosphorylated form of CheY (CheY-P). The binding of CheY-P to a motor is believed to switch the motor's rotational direction from counterclockwise to clockwise. We demonstrated that the rotational switch of a motor was directly regulated by the binding and dissociation of CheY-P by simultaneously visualizing CheY tagged with green fluorescent protein and the rotational switching of a motor in live cells. The binding of 13 ± 7 CheY-P molecules was sufficient to induce clockwise rotation, and CheY-P molecules bound to and dissociated from a motor within ∼100 ms during switching. Thus, we have directly measured the regulation of the output from a signal transduction pathway by intracellular signaling proteins.

Original languageEnglish
Pages (from-to)ra32
JournalScience Signaling
Volume7
Issue number319
DOIs
Publication statusPublished - 2014 Apr 1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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