Microbial diversity in an iron oxidation tank of an AMD treatment plant at an abandoned sulphur mine

Koichi Suto, Hernando Bacosa, Chihiro Inoue, Eiji Matsushima

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper describes about microbial diversity in an iron oxidation tank of an AMD treatment plant established at an abandoned sulphur mine in Japan. Since the mining operation was stopped, this mine has produced strong acidic mine drainage, pH 1.8, including a high concentration of ferrous iron, 301mg/L, and the flow rate was about 4m3/min. In 2006, a pilot scale microbial iron oxidizing system was installed to remove total iron more easily from the AMD by oxidizing ferrous iron to ferric iron. From the start of this pilot operation, microbial diversity in the iron oxidation tank was investigated using a PCR-DGGE method for about two months. In the PCR, V3 region of 16S rRNA gene for Bacteria was amplified. The profiles of DGGE showed that there were three dominant species in the iron oxidation tank through the experimental period. Number of bands on DGGE profiles decreased with dates of sampling so that the microbial population became less diverse because of the iron oxidizing operation. There was a wide variety of bacterial species of even though conditions were strongly acidic.

Original languageEnglish
Title of host publicationBiohydrometallurgy
Subtitle of host publicationFrom the Single Cell to the Environment
PublisherTrans Tech Publications
Pages493-496
Number of pages4
ISBN (Print)0878494529, 9780878494521
DOIs
Publication statusPublished - 2007
Event17th International Biohydrometallurgy Symposium, IBS 2007 - Frankfurt am Main, Germany
Duration: 2007 Sep 22007 Sep 5

Publication series

NameAdvanced Materials Research
Volume20-21
ISSN (Print)1022-6680

Other

Other17th International Biohydrometallurgy Symposium, IBS 2007
CountryGermany
CityFrankfurt am Main
Period07/9/207/9/5

Keywords

  • Acid mine drainage
  • Iron-oxidizing bacteria
  • Microbial diversity
  • PCR-DGGE
  • Treatment

ASJC Scopus subject areas

  • Engineering(all)

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