Microbial community structure of a simultaneous nitrogen and phosphorus removal reactor following treatment in a UASB-DHS system

Masashi Hatamoto, Yayoi Saito, Kazuya Dehama, Nozomi Nakahara, Kyohei Kuroda, Masanobu Takahashi, Takashi Yamaguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The anaerobic-anoxic sequence batch reactor (A2SBR) was applied to achieve nitrogen and phosphorus removal in an energy-saving sewage treatment system involving an up-flow anaerobic sludge blanket combined with a down-flow hanging sponge reactor to treat municipal sewage. After sludge acclimation, the A2SBR showed satisfactory denitrification and phosphorus removal performance with total phosphate and nitrate concentrations of the effluent of 8.4 ± 3.4 mg-N L-1and 0.9 ± 0.6 mg-P L-1, respectively. 16S rRNA gene sequence and fluorescence in situ hybridization analyses revealed that 'Candidatus Accumulibacter phosphatis' was the dominant phosphate-accumulating micro-organism. Although a competitive bacterium for polyphosphate-accumulating organisms, 'Ca. Competibacter phosphatis', was not detected, Dechloromonas spp. were abundant. The ppk1 gene sequence analysis showed that the type II lineage of 'Ca. Accumulibacter' was dominant. The results suggest that denitrification and phosphorus removal in the A2SBR could be achieved by cooperative activity of 'Ca. Accumulibacter' and nitrate-reducing bacteria.

Original languageEnglish
Pages (from-to)454-461
Number of pages8
JournalWater Science and Technology
Volume71
Issue number3
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • A<inf>2</inf>sbr
  • DPAOs
  • Next-generation sequencing
  • Ppk gene
  • UASB-DHS

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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