Microanalysis of Nucleic Acids Using the Limulus G Test

Takahisa Anada, Kazuya Koumoto, Masami Mizu, Ryouji Karinaga, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The limulus G test has been used as a quantitative analysis of (1→3)-β-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1→3)-β-D-glucans or/and polynucleotides on the pico gram/ml scale.

Original languageEnglish
Pages (from-to)1567-1568
Number of pages2
Journalanalytical sciences
Volume19
Issue number12
DOIs
Publication statusPublished - 2003 Dec

ASJC Scopus subject areas

  • Analytical Chemistry

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