Microamperometric estimation of photosynthesis inhibition in a single algal protoplast

Tomokazu Matsue, Shinji Koike, Isamu Uchida

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The single-cell level inhibition of photosynthesis by some chemicals was investigated from the light-irradiation induced response of intracellular oxygen reduction current at an ultramicroelectrode inserted into a single algal protoplast. The addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), an electron-transport inhibitor, decreased a current peak appeared immediately after the light irradiation. The pI50 value (the negative decadic logarithm of the concentration needed for 50 % inhibition) was determined from the change in response patterns and found to be 6.9 for DCMU. 2,4-Dichlorophenoxyacetic acid (2,4-D), an auxin herbicide, showed no effect on photosynthesis in the effective concentration range. 2,4-Dinitrophenol(DNP), a proton carrier, accelerated the oxygen production upon light irradiation.

Original languageEnglish
Pages (from-to)1283-1287
Number of pages5
JournalBiochemical and biophysical research communications
Volume197
Issue number3
DOIs
Publication statusPublished - 1993 Dec 30

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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