Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality

Mami Sumiyoshi; Narumi Masuda; Nobuhiro Tanuma; Honami Ogoh; Eri Imai; Mizuki Otsuka; Natsuki Hayakawa; Kinuyo Ohno; Yasuhisa Matsui; Kanae Hara; Risa Gotoh; Mai Suzuki; Shinya Rai; Hirokazu Tanaka; Itaru Matsumura; Hiroshi Shima; Toshio Watanabe

doi:10.1016/j.febslet.2015.07.050

Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality

Mami Sumiyoshi, Narumi Masuda, Nobuhiro Tanuma, Honami Ogoh, Eri Imai, Mizuki Otsuka, Natsuki Hayakawa, Kinuyo Ohno, Yasuhisa Matsui, Kanae Hara, Risa Gotoh, Mai Suzuki, Shinya Rai, Hirokazu Tanaka, Itaru Matsumura, Hiroshi Shima, Toshio Watanabe

Cell Resource Center for Biomedical Research

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

In mammals, the small Arf GTPase-activating protein (SMAP) subfamily of Arf GTPase-activating proteins consists of closely related members, SMAP1 and SMAP2. These factors reportedly exert distinct functions in membrane trafficking, as manifested by different phenotypes seen in single knockout mice. The present study investigated whether SMAP proteins interact genetically. We report for the first time that simultaneous loss of SMAP1 and SMAP2 promotes apoptosis in the distal region of E7.5 mouse embryos, likely resulting in embryonic lethality. Thus, at least one SMAP gene, either SMAP1 or SMAP2, is required for proper embryogenesis.

Original language	English
Pages (from-to)	2754-2762
Number of pages	9
Journal	FEBS Letters
Volume	589
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2015.07.050
Publication status	Published - 2015 Sep 14

Keywords

Arf GAP
Embryogenesis
Genetic interaction
SMAP1
SMAP2

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2015.07.050

Fingerprint Dive into the research topics of 'Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality'. Together they form a unique fingerprint.

Cite this

Sumiyoshi, M., Masuda, N., Tanuma, N., Ogoh, H., Imai, E., Otsuka, M., Hayakawa, N., Ohno, K., Matsui, Y., Hara, K., Gotoh, R., Suzuki, M., Rai, S., Tanaka, H., Matsumura, I., Shima, H., & Watanabe, T. (2015). Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality. FEBS Letters, 589(19), 2754-2762. https://doi.org/10.1016/j.febslet.2015.07.050

Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality. / Sumiyoshi, Mami; Masuda, Narumi; Tanuma, Nobuhiro; Ogoh, Honami; Imai, Eri; Otsuka, Mizuki; Hayakawa, Natsuki; Ohno, Kinuyo; Matsui, Yasuhisa; Hara, Kanae; Gotoh, Risa; Suzuki, Mai; Rai, Shinya; Tanaka, Hirokazu; Matsumura, Itaru; Shima, Hiroshi; Watanabe, Toshio.

In: FEBS Letters, Vol. 589, No. 19, 14.09.2015, p. 2754-2762.

Research output: Contribution to journal › Article › peer-review

Sumiyoshi, Mami ; Masuda, Narumi ; Tanuma, Nobuhiro ; Ogoh, Honami ; Imai, Eri ; Otsuka, Mizuki ; Hayakawa, Natsuki ; Ohno, Kinuyo ; Matsui, Yasuhisa ; Hara, Kanae ; Gotoh, Risa ; Suzuki, Mai ; Rai, Shinya ; Tanaka, Hirokazu ; Matsumura, Itaru ; Shima, Hiroshi ; Watanabe, Toshio. / Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality. In: FEBS Letters. 2015 ; Vol. 589, No. 19. pp. 2754-2762.

@article{e158de86a3ca42358c2824bfb1633d36,

title = "Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality",

abstract = "In mammals, the small Arf GTPase-activating protein (SMAP) subfamily of Arf GTPase-activating proteins consists of closely related members, SMAP1 and SMAP2. These factors reportedly exert distinct functions in membrane trafficking, as manifested by different phenotypes seen in single knockout mice. The present study investigated whether SMAP proteins interact genetically. We report for the first time that simultaneous loss of SMAP1 and SMAP2 promotes apoptosis in the distal region of E7.5 mouse embryos, likely resulting in embryonic lethality. Thus, at least one SMAP gene, either SMAP1 or SMAP2, is required for proper embryogenesis.",

keywords = "Arf GAP, Embryogenesis, Genetic interaction, SMAP1, SMAP2",

author = "Mami Sumiyoshi and Narumi Masuda and Nobuhiro Tanuma and Honami Ogoh and Eri Imai and Mizuki Otsuka and Natsuki Hayakawa and Kinuyo Ohno and Yasuhisa Matsui and Kanae Hara and Risa Gotoh and Mai Suzuki and Shinya Rai and Hirokazu Tanaka and Itaru Matsumura and Hiroshi Shima and Toshio Watanabe",

year = "2015",

month = sep,

day = "14",

doi = "10.1016/j.febslet.2015.07.050",

language = "English",

volume = "589",

pages = "2754--2762",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "19",

}

TY - JOUR

T1 - Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality

AU - Sumiyoshi, Mami

AU - Masuda, Narumi

AU - Tanuma, Nobuhiro

AU - Ogoh, Honami

AU - Imai, Eri

AU - Otsuka, Mizuki

AU - Hayakawa, Natsuki

AU - Ohno, Kinuyo

AU - Matsui, Yasuhisa

AU - Hara, Kanae

AU - Gotoh, Risa

AU - Suzuki, Mai

AU - Rai, Shinya

AU - Tanaka, Hirokazu

AU - Matsumura, Itaru

AU - Shima, Hiroshi

AU - Watanabe, Toshio

PY - 2015/9/14

Y1 - 2015/9/14

N2 - In mammals, the small Arf GTPase-activating protein (SMAP) subfamily of Arf GTPase-activating proteins consists of closely related members, SMAP1 and SMAP2. These factors reportedly exert distinct functions in membrane trafficking, as manifested by different phenotypes seen in single knockout mice. The present study investigated whether SMAP proteins interact genetically. We report for the first time that simultaneous loss of SMAP1 and SMAP2 promotes apoptosis in the distal region of E7.5 mouse embryos, likely resulting in embryonic lethality. Thus, at least one SMAP gene, either SMAP1 or SMAP2, is required for proper embryogenesis.

AB - In mammals, the small Arf GTPase-activating protein (SMAP) subfamily of Arf GTPase-activating proteins consists of closely related members, SMAP1 and SMAP2. These factors reportedly exert distinct functions in membrane trafficking, as manifested by different phenotypes seen in single knockout mice. The present study investigated whether SMAP proteins interact genetically. We report for the first time that simultaneous loss of SMAP1 and SMAP2 promotes apoptosis in the distal region of E7.5 mouse embryos, likely resulting in embryonic lethality. Thus, at least one SMAP gene, either SMAP1 or SMAP2, is required for proper embryogenesis.

KW - Arf GAP

KW - Embryogenesis

KW - Genetic interaction

KW - SMAP1

KW - SMAP2

UR - http://www.scopus.com/inward/record.url?scp=84941366482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941366482&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2015.07.050

DO - 10.1016/j.febslet.2015.07.050

M3 - Article

C2 - 26296315

AN - SCOPUS:84941366482

VL - 589

SP - 2754

EP - 2762

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 19

ER -