Mice deficient in Dmrt7 show infertility with spermatogenic arrest at pachytene stage

Masaki Kawamata; Katsuhiko Nishimori

doi:10.1016/j.febslet.2006.10.066

Mice deficient in Dmrt7 show infertility with spermatogenic arrest at pachytene stage

Masaki Kawamata, Katsuhiko Nishimori

AGR - Life Sciences

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Genes including DM domain regulate sexual development in diverse metazoan phyla. One of these genes, Dmrt7, was expressed only in testes of adult mice. To determine the role of Dmrt7 in mice, we generated Dmrt7-knockout mice (Dmrt7^-/-). Although the Dmrt7^-/- showed normal growth, null males were infertile. No sperm was detected in the epididymis of Dmrt7^-/- adult males. Absence of spermatids in a histological analysis, decreased expression of Ccna1 mRNA and the accumulation of SCP3-positive spermatocytes showed the arrest of spermatogenesis at the pachytene stage in the Dmrt7-knockout mice.

Original language	English
Pages (from-to)	6442-6446
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2006.10.066
Publication status	Published - 2006 Nov 27

Keywords

Dmrt7
Knockout mouse
Male infertility
Spermatogenesis
Testis

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Mice deficient in Dmrt7 show infertility with spermatogenic arrest at pachytene stage",

abstract = "Genes including DM domain regulate sexual development in diverse metazoan phyla. One of these genes, Dmrt7, was expressed only in testes of adult mice. To determine the role of Dmrt7 in mice, we generated Dmrt7-knockout mice (Dmrt7-/-). Although the Dmrt7-/- showed normal growth, null males were infertile. No sperm was detected in the epididymis of Dmrt7-/- adult males. Absence of spermatids in a histological analysis, decreased expression of Ccna1 mRNA and the accumulation of SCP3-positive spermatocytes showed the arrest of spermatogenesis at the pachytene stage in the Dmrt7-knockout mice.",

keywords = "Dmrt7, Knockout mouse, Male infertility, Spermatogenesis, Testis",

author = "Masaki Kawamata and Katsuhiko Nishimori",

note = "Funding Information: We thank S. Jamin and R.R. Behringer for IRES-Cre-pA-FRT-PGK-Neo-pA-FRT plasmid and N. Nakatsuji and S. Chuma for anti-SCP3 antibody. This work was supported in part by Grants-in-Aid for Scientific Research (No. 16108003) from the Ministry of Education, Science and Culture of Japan. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2006",

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doi = "10.1016/j.febslet.2006.10.066",

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AU - Nishimori, Katsuhiko

N1 - Funding Information: We thank S. Jamin and R.R. Behringer for IRES-Cre-pA-FRT-PGK-Neo-pA-FRT plasmid and N. Nakatsuji and S. Chuma for anti-SCP3 antibody. This work was supported in part by Grants-in-Aid for Scientific Research (No. 16108003) from the Ministry of Education, Science and Culture of Japan. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2006/11/27

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N2 - Genes including DM domain regulate sexual development in diverse metazoan phyla. One of these genes, Dmrt7, was expressed only in testes of adult mice. To determine the role of Dmrt7 in mice, we generated Dmrt7-knockout mice (Dmrt7-/-). Although the Dmrt7-/- showed normal growth, null males were infertile. No sperm was detected in the epididymis of Dmrt7-/- adult males. Absence of spermatids in a histological analysis, decreased expression of Ccna1 mRNA and the accumulation of SCP3-positive spermatocytes showed the arrest of spermatogenesis at the pachytene stage in the Dmrt7-knockout mice.

AB - Genes including DM domain regulate sexual development in diverse metazoan phyla. One of these genes, Dmrt7, was expressed only in testes of adult mice. To determine the role of Dmrt7 in mice, we generated Dmrt7-knockout mice (Dmrt7-/-). Although the Dmrt7-/- showed normal growth, null males were infertile. No sperm was detected in the epididymis of Dmrt7-/- adult males. Absence of spermatids in a histological analysis, decreased expression of Ccna1 mRNA and the accumulation of SCP3-positive spermatocytes showed the arrest of spermatogenesis at the pachytene stage in the Dmrt7-knockout mice.

KW - Dmrt7

KW - Knockout mouse

KW - Male infertility

KW - Spermatogenesis

KW - Testis

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Mice deficient in Dmrt7 show infertility with spermatogenic arrest at pachytene stage

Abstract

Keywords

ASJC Scopus subject areas

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