MgLig4, a homolog of Neurospora crassa Mus-53 (DNA ligase IV), is involved in, but not essential for, non-homologous end-joining events in Magnaporthe grisea

Hideki Kito, Takashi Fujikawa, Akihiro Moriwaki, Ayami Tomono, Masumi Izawa, Takashi Kamakura, Miho Ohashi, Hiroyoshi Sato, Keietsu Abe, Marie Nishimura

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

In many eukaryotic organisms, the non-homologous end-joining (NHEJ) system is a major pathway for the repair of DNA double-strand breaks (DSBs). DNA ligase IV is a component of the NHEJ system and is strictly required for the NHEJ system in Saccharomyces cerevisiae and in Neurospora crassa. To investigate the functions of DNA Ligase IV in Magnaporthe grisea, we generated deletion mutants of MGLIG4, which encodes a homolog of N. crassa DNA Ligase IV. Mutants (mglig4) showed no defects in asexual or sexual growth, and were fully pathogenic. Compared to the wild-type, mglig4 exhibited weak sensitivity to a DNA-damaging agent, camptothecin. In addition, the frequency of targeted-gene replacement was relatively elevated in mglig4, although this varied in a gene-dependent manner. Surprisingly, non-homologous integration of DNA was frequently observed in mglig4 transformants. Our results demonstrate that MgLig4 is involved in, but not essential for, the NHEJ system in M. grisea.

Original languageEnglish
Pages (from-to)1543-1551
Number of pages9
JournalFungal Genetics and Biology
Volume45
Issue number12
DOIs
Publication statusPublished - 2008 Dec

Keywords

  • DNA ligase IV
  • Gene targeting
  • Magnaporthe grisea
  • Non-homologous end-joining

ASJC Scopus subject areas

  • Microbiology
  • Genetics

Fingerprint Dive into the research topics of 'MgLig4, a homolog of Neurospora crassa Mus-53 (DNA ligase IV), is involved in, but not essential for, non-homologous end-joining events in Magnaporthe grisea'. Together they form a unique fingerprint.

Cite this