Methylglyoxal induces apoptosis in Jurkat leukemia T cells by activating c-jun N-terminal kinase

Jun Du, Haruhiko Suzuki, Fumihiko Nagase, Anwarul A. Akhand, Toshihiro Yokoyama, Toshio Miyata, Kiyoshi Kurokawa, Izumi Nakashima

Research output: Contribution to journalArticlepeer-review

94 Citations (Scopus)

Abstract

Methylglyoxal (MG) is a physiological metabolite, but it is known to be toxic, inducing stress in cells and causing apoptosis. This study examines molecular mechanisms in the MG-induced signal transduction leading to apoptosis, focusing particularly on the role of JNK activation. We first confirmed that MG caused apoptosis in Jurkat cells and that it was cell type dependent because it failed to induce apoptosis in MOLT-4, HeLa, or COS-7 cells. A caspase inhibitor, Z-DEVD-fmk, completely blocked MG-induced poly(ADP-ribose)polymerase (PARP) cleavage and apoptosis, showing the critical role of caspase activation. Inhibition of JNK activity by a JNK inhibitor, curcumin, remarkably reduced MG-induced caspase-3 activation, PARP cleavage, and apoptosis. Stable expression of the dominant negative mutant of JNK also protected cells against apoptosis notably, although not completely. Correspondingly, loss of the mitochondrial membrane potential induced by MG was decreased by the dominant negative JNK. These results confirmed a crucial role of JNK working upstream of caspases, as well as an involvement of JNK in affecting the mitochondrial membrane potential.

Original languageEnglish
Pages (from-to)333-344
Number of pages12
JournalJournal of Cellular Biochemistry
Volume77
Issue number2
DOIs
Publication statusPublished - 2000 Apr 11
Externally publishedYes

Keywords

  • Apoptosis
  • Caspase
  • JNK
  • Jurkat
  • Methylglyoxal

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Methylglyoxal induces apoptosis in Jurkat leukemia T cells by activating c-jun N-terminal kinase'. Together they form a unique fingerprint.

Cite this