Methods of analysis of the membrane trafficking pathway from recycling endosomes to lysosomes

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The transferrin receptor (TfR) is responsible for iron uptake through its trafficking between the plasma membrane and recycling endosomes, and as a result it has become a well-known marker for recycling endosomes. Although the molecular basis of the TfR recycling pathway has been thoroughly investigated, the TfR degradation mechanism has been poorly understood. Exposure of cultured cells to two drugs, the protein synthesis inhibitor cycloheximide and the V-ATPase inhibitor bafilomycin A1, recently showed that TfR is not only recycled back to the plasma membrane after endocytosis but is constitutively transported to lysosomes for degradation. The results of genome-wide screening of mouse Rab small GTPases (common regulators of membrane trafficking in all eukaryotes) have indicated that Rab12 regulates TfR trafficking to lysosomes independently of the known membrane trafficking pathways, for example, the conventional endocytic pathway and recycling pathway. This chapter summarizes the methods that the authors used to analyze the membrane trafficking pathway from recycling endosomes to lysosomes that is specifically regulated by Rab12.

Original languageEnglish
Title of host publicationEndosome Signaling Part A
PublisherAcademic Press Inc.
Pages195-206
Number of pages12
ISBN (Print)9780123979261
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Enzymology
Volume534
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Degradation
  • Lysosome
  • Membrane traffic
  • Rab12
  • Recycling endosome
  • Small GTPase
  • Transferrin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Methods of analysis of the membrane trafficking pathway from recycling endosomes to lysosomes'. Together they form a unique fingerprint.

Cite this