Methionine-enkephalin-Arg6-Gly7-Leu8-immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat: An immunohistochemical study

Tooru Shimosegawa; Masaru Koizumi; Takayoshi Toyota; Yoshio Goto; Shigeru Kobayashi; Chizuko Yanaihara; Noboru Yanaihara

doi:10.1016/0304-3940(85)90058-8

Methionine-enkephalin-Arg⁶-Gly⁷-Leu⁸-immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat: An immunohistochemical study

Tooru Shimosegawa, Masaru Koizumi, Takayoshi Toyota, Yoshio Goto, Shigeru Kobayashi, Chizuko Yanaihara, Noboru Yanaihara

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Lumbar paravertebral ganglia and prevertebral ganglia of the rat were immunohistochemically examined using an antiserum against Methionine-enkephalin-Arg⁶-Gly⁷-Leu⁸ (Met-Enk-Arg-Gly-Leu). Colchicine (5 mg/kg body wt., i.p. injection) was employed as an axonal transport blocker to intensify the immunoreactivity in the nerve cell bodies. In the lumbar paravertebral ganglia of the colchicine-treated rats, about 55% of nerve cell bodies showed a positive immunoreactivity. In the celiac-superior mesenteric ganglion complex, a dense network of positively immunostained nerve fiber varicosities surrounded immunonegative nerve cell bodies. The Met-Enk-Arg-Gly-Leu-like immunoreactivity demonstrated in this study suggests the occurrence of preproenkephalin A and related opioid peptides.

Original language	English
Pages (from-to)	169-174
Number of pages	6
Journal	Neuroscience Letters
Volume	57
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(85)90058-8
Publication status	Published - 1985 Jun 12

Keywords

celiac-superior mesenteric ganglion complex
colchicine
immunohistochemistry
lumbar paravertebral ganglion
methionine-enkephalin-Arg-Gly-Leu
rat

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(85)90058-8

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Shimosegawa, T., Koizumi, M., Toyota, T., Goto, Y., Kobayashi, S., Yanaihara, C., & Yanaihara, N. (1985). Methionine-enkephalin-Arg⁶-Gly⁷-Leu⁸-immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat: An immunohistochemical study. Neuroscience Letters, 57(2), 169-174. https://doi.org/10.1016/0304-3940(85)90058-8

Methionine-enkephalin-Arg⁶-Gly⁷-Leu⁸-immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat : An immunohistochemical study. / Shimosegawa, Tooru; Koizumi, Masaru; Toyota, Takayoshi; Goto, Yoshio; Kobayashi, Shigeru; Yanaihara, Chizuko; Yanaihara, Noboru.

In: Neuroscience Letters, Vol. 57, No. 2, 12.06.1985, p. 169-174.

Research output: Contribution to journal › Article › peer-review

Shimosegawa, T, Koizumi, M, Toyota, T, Goto, Y, Kobayashi, S, Yanaihara, C & Yanaihara, N 1985, 'Methionine-enkephalin-Arg⁶-Gly⁷-Leu⁸-immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat: An immunohistochemical study', Neuroscience Letters, vol. 57, no. 2, pp. 169-174. https://doi.org/10.1016/0304-3940(85)90058-8

Shimosegawa T, Koizumi M, Toyota T, Goto Y, Kobayashi S, Yanaihara C et al. Methionine-enkephalin-Arg⁶-Gly⁷-Leu⁸-immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat: An immunohistochemical study. Neuroscience Letters. 1985 Jun 12;57(2):169-174. https://doi.org/10.1016/0304-3940(85)90058-8

Shimosegawa, Tooru ; Koizumi, Masaru ; Toyota, Takayoshi ; Goto, Yoshio ; Kobayashi, Shigeru ; Yanaihara, Chizuko ; Yanaihara, Noboru. / Methionine-enkephalin-Arg⁶-Gly⁷-Leu⁸-immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat : An immunohistochemical study. In: Neuroscience Letters. 1985 ; Vol. 57, No. 2. pp. 169-174.

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abstract = "Lumbar paravertebral ganglia and prevertebral ganglia of the rat were immunohistochemically examined using an antiserum against Methionine-enkephalin-Arg6-Gly7-Leu8 (Met-Enk-Arg-Gly-Leu). Colchicine (5 mg/kg body wt., i.p. injection) was employed as an axonal transport blocker to intensify the immunoreactivity in the nerve cell bodies. In the lumbar paravertebral ganglia of the colchicine-treated rats, about 55% of nerve cell bodies showed a positive immunoreactivity. In the celiac-superior mesenteric ganglion complex, a dense network of positively immunostained nerve fiber varicosities surrounded immunonegative nerve cell bodies. The Met-Enk-Arg-Gly-Leu-like immunoreactivity demonstrated in this study suggests the occurrence of preproenkephalin A and related opioid peptides.",

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