TY - JOUR
T1 - Methionine-enkephalin-Arg6-Gly7-Leu8-immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat
T2 - An immunohistochemical study
AU - Shimosegawa, Tooru
AU - Koizumi, Masaru
AU - Toyota, Takayoshi
AU - Goto, Yoshio
AU - Kobayashi, Shigeru
AU - Yanaihara, Chizuko
AU - Yanaihara, Noboru
PY - 1985/6/12
Y1 - 1985/6/12
N2 - Lumbar paravertebral ganglia and prevertebral ganglia of the rat were immunohistochemically examined using an antiserum against Methionine-enkephalin-Arg6-Gly7-Leu8 (Met-Enk-Arg-Gly-Leu). Colchicine (5 mg/kg body wt., i.p. injection) was employed as an axonal transport blocker to intensify the immunoreactivity in the nerve cell bodies. In the lumbar paravertebral ganglia of the colchicine-treated rats, about 55% of nerve cell bodies showed a positive immunoreactivity. In the celiac-superior mesenteric ganglion complex, a dense network of positively immunostained nerve fiber varicosities surrounded immunonegative nerve cell bodies. The Met-Enk-Arg-Gly-Leu-like immunoreactivity demonstrated in this study suggests the occurrence of preproenkephalin A and related opioid peptides.
AB - Lumbar paravertebral ganglia and prevertebral ganglia of the rat were immunohistochemically examined using an antiserum against Methionine-enkephalin-Arg6-Gly7-Leu8 (Met-Enk-Arg-Gly-Leu). Colchicine (5 mg/kg body wt., i.p. injection) was employed as an axonal transport blocker to intensify the immunoreactivity in the nerve cell bodies. In the lumbar paravertebral ganglia of the colchicine-treated rats, about 55% of nerve cell bodies showed a positive immunoreactivity. In the celiac-superior mesenteric ganglion complex, a dense network of positively immunostained nerve fiber varicosities surrounded immunonegative nerve cell bodies. The Met-Enk-Arg-Gly-Leu-like immunoreactivity demonstrated in this study suggests the occurrence of preproenkephalin A and related opioid peptides.
KW - celiac-superior mesenteric ganglion complex
KW - colchicine
KW - immunohistochemistry
KW - lumbar paravertebral ganglion
KW - methionine-enkephalin-Arg-Gly-Leu
KW - rat
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U2 - 10.1016/0304-3940(85)90058-8
DO - 10.1016/0304-3940(85)90058-8
M3 - Article
C2 - 3897912
AN - SCOPUS:0021891428
VL - 57
SP - 169
EP - 174
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -