Abstract
We studied the relationship between the metastatic potential of murine B16 melanoma cells and their surface expression of heparan sulfate glycosaminoglycan (HS-GAG) by using HepSS-1, a monoclonal antibody specific to HS-GAG. Firstly, among five B16 sublines, those capable of developing lung colonies with high efficiencies, such as B16-F10, had relatively low levels of surface HS-GAG. Secondly, a subline freshly prepared from metastatic lung colonies (Fl) displayed a level of surface HS-GAG lower than that of injected B16 cells. Thirdly, in vitro selection of B16 cells with low surface HS-GAG by repeated HepSS-1 staining and cell-sorting resulted in cells with a higher metastatic efficiency than that of the original B16 cells. Collectively, B16 melanoma cells with high metastatic activities seem to have low surface HS-GAG. We also found that there was a positive correlation between the surface level of HS-GAG and the susceptibility to natural killer cells in eight B16 sublines.
| Original language | English |
|---|---|
| Pages (from-to) | 1238-1245 |
| Number of pages | 8 |
| Journal | Japanese Journal of Cancer Research GANN |
| Volume | 78 |
| Issue number | 11 |
| Publication status | Published - 1987 Jan 1 |
Keywords
- B16 melanoma
- Heparan sulfate
- Metastasis
- Monoclonal antibody
ASJC Scopus subject areas
- Oncology
- Cancer Research
