Metastatic Potential of Murine B16 Melanoma Correlates with Reduced Surface Heparan Sulfate Glycosaminoglycan

Shigeo Kure, Osamu Yoshie, Hisashi Aso

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18 Citations (Scopus)

Abstract

We studied the relationship between the metastatic potential of murine B16 melanoma cells and their surface expression of heparan sulfate glycosaminoglycan (HS-GAG) by using HepSS-1, a monoclonal antibody specific to HS-GAG. Firstly, among five B16 sublines, those capable of developing lung colonies with high efficiencies, such as B16-F10, had relatively low levels of surface HS-GAG. Secondly, a subline freshly prepared from metastatic lung colonies (Fl) displayed a level of surface HS-GAG lower than that of injected B16 cells. Thirdly, in vitro selection of B16 cells with low surface HS-GAG by repeated HepSS-1 staining and cell-sorting resulted in cells with a higher metastatic efficiency than that of the original B16 cells. Collectively, B16 melanoma cells with high metastatic activities seem to have low surface HS-GAG. We also found that there was a positive correlation between the surface level of HS-GAG and the susceptibility to natural killer cells in eight B16 sublines.

Original languageEnglish
Pages (from-to)1238-1245
Number of pages8
JournalJapanese Journal of Cancer Research GANN
Volume78
Issue number11
Publication statusPublished - 1987 Jan 1

Keywords

  • B16 melanoma
  • Heparan sulfate
  • Metastasis
  • Monoclonal antibody

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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