Metallothionein mediates gene expression of 3.1 mRNA (PTZ17) related to epileptic seizure

Nobuhiko Miura, Akira Naganuma

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Genes differentially expressed in association with disruption of the metallothionein gene were screened using two hepatic stellate cell lines isolated and established from the livers of normal 129/Sv (IMS/N cells) and transgenic mice deficient in the genes for metallothionein-I and -II (IMS/MT (-) cells). We found one cDNA (tentatively named NM31) that was expressed only in IMS/N cells. Transfecting IMS/MT (-) cells with the genes for both metallothionein-I and -II resulted in NM31 expression. These results suggest that metallothionein is essential for NM31 gene expression. The nucleotide sequence of NM31 (294 bp) was identical to the 3' region of 3.1 mRNA (PTZ 17), which is abundant in the embryonic mouse brain and is related to chemically induced seizures. The present study indicates that metallothionein mediates the expression of specific genes. This is a novel explanation for some of the functions of metallothionein. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)146-148
Number of pages3
JournalFEBS Letters
Issue number3
Publication statusPublished - 2000 Aug 18


  • 3.1 mRNA
  • Gene expression
  • Metallothionein
  • NM31
  • PTZ 17
  • Stellate cell

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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