Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS

Satomi Kameo, Kunihiko Nakai, Naoyuki Kurokawa, Tomokazu Kanehisa, Akira Naganuma, Hiroshi Satoh

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The influences of the lack of metallothionein (MT)-I and MT-II on mercury accumulation in the brain and the changes of zinc and copper concentrations and metal components of MTs were examined after mercury vapor exposure by using MT-I, II null mice and 129/Sv mice as experimental animals. Metal components of metal-binding proteins of soluble fractions in the brain sections were analyzed by high-performance liquid chromatography (HPLC) connected with inductively coupled plasma mass spectrometry (ICP-MS). The Hg levels of MT-I, II null mice in each brain section were significantly higher than those of wild-type mice after mercury vapor exposure. It was suggested that MT-III is associated with the storage of mercury in conditions lacking MT-I and MT-II.

Original languageEnglish
Pages (from-to)1514-1519
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume381
Issue number8
DOIs
Publication statusPublished - 2005 Apr

Keywords

  • Brain
  • Copper
  • ICP-MS
  • Mercury vapor
  • Metallothionein
  • Zinc

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Fingerprint Dive into the research topics of 'Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS'. Together they form a unique fingerprint.

Cite this