Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS

Satomi Kameo; Kunihiko Nakai; Naoyuki Kurokawa; Tomokazu Kanehisa; Akira Naganuma; Hiroshi Satoh

doi:10.1007/s00216-005-3157-9

Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS

Satomi Kameo, Kunihiko Nakai, Naoyuki Kurokawa, Tomokazu Kanehisa, Akira Naganuma, Hiroshi Satoh

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The influences of the lack of metallothionein (MT)-I and MT-II on mercury accumulation in the brain and the changes of zinc and copper concentrations and metal components of MTs were examined after mercury vapor exposure by using MT-I, II null mice and 129/Sv mice as experimental animals. Metal components of metal-binding proteins of soluble fractions in the brain sections were analyzed by high-performance liquid chromatography (HPLC) connected with inductively coupled plasma mass spectrometry (ICP-MS). The Hg levels of MT-I, II null mice in each brain section were significantly higher than those of wild-type mice after mercury vapor exposure. It was suggested that MT-III is associated with the storage of mercury in conditions lacking MT-I and MT-II.

Original language	English
Pages (from-to)	1514-1519
Number of pages	6
Journal	Analytical and Bioanalytical Chemistry
Volume	381
Issue number	8
DOIs	https://doi.org/10.1007/s00216-005-3157-9
Publication status	Published - 2005 Apr

Keywords

Brain
Copper
ICP-MS
Mercury vapor
Metallothionein
Zinc

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS. / Kameo, Satomi; Nakai, Kunihiko; Kurokawa, Naoyuki; Kanehisa, Tomokazu; Naganuma, Akira; Satoh, Hiroshi.

In: Analytical and Bioanalytical Chemistry, Vol. 381, No. 8, 04.2005, p. 1514-1519.

Research output: Contribution to journal › Article › peer-review

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abstract = "The influences of the lack of metallothionein (MT)-I and MT-II on mercury accumulation in the brain and the changes of zinc and copper concentrations and metal components of MTs were examined after mercury vapor exposure by using MT-I, II null mice and 129/Sv mice as experimental animals. Metal components of metal-binding proteins of soluble fractions in the brain sections were analyzed by high-performance liquid chromatography (HPLC) connected with inductively coupled plasma mass spectrometry (ICP-MS). The Hg levels of MT-I, II null mice in each brain section were significantly higher than those of wild-type mice after mercury vapor exposure. It was suggested that MT-III is associated with the storage of mercury in conditions lacking MT-I and MT-II.",

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