Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS

Satomi Kameo; Kunihiko Nakai; Naoyuki Kurokawa; Tomokazu Kanehisa; Akira Naganuma; Hiroshi Satoh

doi:10.1007/s00216-005-3157-9

Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS

Satomi Kameo, Kunihiko Nakai, Naoyuki Kurokawa, Tomokazu Kanehisa, Akira Naganuma, Hiroshi Satoh

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The influences of the lack of metallothionein (MT)-I and MT-II on mercury accumulation in the brain and the changes of zinc and copper concentrations and metal components of MTs were examined after mercury vapor exposure by using MT-I, II null mice and 129/Sv mice as experimental animals. Metal components of metal-binding proteins of soluble fractions in the brain sections were analyzed by high-performance liquid chromatography (HPLC) connected with inductively coupled plasma mass spectrometry (ICP-MS). The Hg levels of MT-I, II null mice in each brain section were significantly higher than those of wild-type mice after mercury vapor exposure. It was suggested that MT-III is associated with the storage of mercury in conditions lacking MT-I and MT-II.

Original language	English
Pages (from-to)	1514-1519
Number of pages	6
Journal	Analytical and Bioanalytical Chemistry
Volume	381
Issue number	8
DOIs	https://doi.org/10.1007/s00216-005-3157-9
Publication status	Published - 2005 Apr
Externally published	Yes

Keywords

Brain
Copper
ICP-MS
Mercury vapor
Metallothionein
Zinc

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1007/s00216-005-3157-9

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Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS. / Kameo, Satomi; Nakai, Kunihiko; Kurokawa, Naoyuki et al.

In: Analytical and Bioanalytical Chemistry, Vol. 381, No. 8, 04.2005, p. 1514-1519.

Research output: Contribution to journal › Article › peer-review

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title = "Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS",

abstract = "The influences of the lack of metallothionein (MT)-I and MT-II on mercury accumulation in the brain and the changes of zinc and copper concentrations and metal components of MTs were examined after mercury vapor exposure by using MT-I, II null mice and 129/Sv mice as experimental animals. Metal components of metal-binding proteins of soluble fractions in the brain sections were analyzed by high-performance liquid chromatography (HPLC) connected with inductively coupled plasma mass spectrometry (ICP-MS). The Hg levels of MT-I, II null mice in each brain section were significantly higher than those of wild-type mice after mercury vapor exposure. It was suggested that MT-III is associated with the storage of mercury in conditions lacking MT-I and MT-II.",

keywords = "Brain, Copper, ICP-MS, Mercury vapor, Metallothionein, Zinc",

author = "Satomi Kameo and Kunihiko Nakai and Naoyuki Kurokawa and Tomokazu Kanehisa and Akira Naganuma and Hiroshi Satoh",

note = "Funding Information: Acknowledgements This work was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology. The study was carried out in accordance with the Guide of Animal Experimentation, Tohoku University and Tohoku University School of Medicine. The authors thank Chieko Inomata and Sato Yashima for their excellent technical assistance.",

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AU - Kameo, Satomi

AU - Nakai, Kunihiko

AU - Kurokawa, Naoyuki

AU - Kanehisa, Tomokazu

AU - Naganuma, Akira

AU - Satoh, Hiroshi

N1 - Funding Information: Acknowledgements This work was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology. The study was carried out in accordance with the Guide of Animal Experimentation, Tohoku University and Tohoku University School of Medicine. The authors thank Chieko Inomata and Sato Yashima for their excellent technical assistance.

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N2 - The influences of the lack of metallothionein (MT)-I and MT-II on mercury accumulation in the brain and the changes of zinc and copper concentrations and metal components of MTs were examined after mercury vapor exposure by using MT-I, II null mice and 129/Sv mice as experimental animals. Metal components of metal-binding proteins of soluble fractions in the brain sections were analyzed by high-performance liquid chromatography (HPLC) connected with inductively coupled plasma mass spectrometry (ICP-MS). The Hg levels of MT-I, II null mice in each brain section were significantly higher than those of wild-type mice after mercury vapor exposure. It was suggested that MT-III is associated with the storage of mercury in conditions lacking MT-I and MT-II.

AB - The influences of the lack of metallothionein (MT)-I and MT-II on mercury accumulation in the brain and the changes of zinc and copper concentrations and metal components of MTs were examined after mercury vapor exposure by using MT-I, II null mice and 129/Sv mice as experimental animals. Metal components of metal-binding proteins of soluble fractions in the brain sections were analyzed by high-performance liquid chromatography (HPLC) connected with inductively coupled plasma mass spectrometry (ICP-MS). The Hg levels of MT-I, II null mice in each brain section were significantly higher than those of wild-type mice after mercury vapor exposure. It was suggested that MT-III is associated with the storage of mercury in conditions lacking MT-I and MT-II.

KW - Brain

KW - Copper

KW - ICP-MS

KW - Mercury vapor

KW - Metallothionein

KW - Zinc

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Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS

Abstract

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