Metabolomics of supragingival plaque and oral bacteria

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

Dental caries is initiated by demineralization of the tooth surface through acid production by sugar metabolism of supragingival plaque microflora. To elucidate the sugar metabolic system, we used CE-MS to perform metabolomics of the central carbon metabolism, the EMP pathway, the pentose-phosphate pathway, and the TCA cycle in supragingival plaque and representative oral bacteria, Streptococcus and Actinomyces. Supragingival plaque contained all the targeted metabolites in the central carbon metabolism, except erythrose 4-phosphate in the pentose-phosphate pathway. After glucose rinse, glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, dihydroxyacetone phosphate, and pyruvate in the EMP pathway and 6-phosphogluconate, ribulose 5-phosphate, and sedoheptulose 7-phosphate in the pentose-phosphate pathway, and acetyl CoA were increased. Meanwhile, 3-phosphoglycerate and phosphoenolpyruvate in the EMP pathway and succinate, fumarate, and malate in the TCA cycle were decreased. These pathways and changes in metabolites observed in supragingival plaque were similar to the integration of metabolite profiles in Streptococcus and Actinomyces.

Original languageEnglish
Pages (from-to)1383-1388
Number of pages6
JournalJournal of dental research
Volume89
Issue number12
DOIs
Publication statusPublished - 2010 Dec

Keywords

  • Actinomyces
  • Streptococcus
  • metabolomics
  • plaque
  • sugar metabolism

ASJC Scopus subject areas

  • Dentistry(all)

Fingerprint

Dive into the research topics of 'Metabolomics of supragingival plaque and oral bacteria'. Together they form a unique fingerprint.

Cite this