Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells

Yohei Hayashi, Yasuhisa Matsui

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Primordial germ cells (PGCs), the precursors of gametes, are the only cells capable of acquiring totipotency upon fertilization, but the molecular mechanisms regulating germ cell characteristics have not been fully elucidated. Although intracellular metabolic status and regulation are responsible for the control of cell function and differentiation, little is known about the metabolic features of PGCs. Here, we describe use of an integrated metabolomic, proteomic, and energy metabolic analysis method to comprehensively elucidate the metabolic characteristics of PGCs using mass spectrometry.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages259-269
Number of pages11
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
Volume2045
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Glycolysis
  • Metabolome
  • Oxidative phosphorylation
  • Primordial germ cells
  • Proteome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Hayashi, Y., & Matsui, Y. (2019). Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells. In Methods in Molecular Biology (pp. 259-269). (Methods in Molecular Biology; Vol. 2045). Humana Press Inc.. https://doi.org/10.1007/7651_2018_164