Metabolism and cytotoxic effects of phosphatidylcholine hydroperoxide in human hepatoma HepG2 cells

Yuuri Suzuki, Kiyotaka Nakagawa, Shunji Kato, Naoto Tatewaki, Shunsuke Mizuochi, Junya Ito, Takahiro Eitsuka, Hiroshi Nishida, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Abstract In this study, we investigated cellular uptake and metabolism of phosphatidylcholine hydroperoxide (PCOOH) in human hepatoma HepG2 cells by high performance liquid chromatography-tandem mass spectrometry, and then evaluated whether PCOOH or its metabolites cause pathophysiological effects such as cytotoxicity and apoptosis. Although we found that most PCOOH was reduced to PC hydroxide in HepG2 cells, the remaining PCOOH caused cytotoxic effects that may be mediated through an unusual apoptosis pathway. These results will enhance our fundamental understanding of how PCOOH, which is present in oxidized low density lipoproteins, is involved in the development of atherosclerosis.

Original languageEnglish
Article number33455
Pages (from-to)920-927
Number of pages8
JournalBiochemical and biophysical research communications
Volume458
Issue number4
DOIs
Publication statusPublished - 2015 Mar 20

Keywords

  • Atherosclerosis
  • Cytotoxic effects
  • Human hepatoma HepG2 cells
  • Metabolism
  • Phosphatidylcholine hydroperoxide
  • Unusual apoptosis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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