Metabolic suppression during mesodermal differentiation of embryonic stem cells identified by single-cell comprehensive gene expression analysis

Yuanshu Zhou, Ikuma Fujisawa, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Flk-1 (VEGF receptor 2) is a well-defined mesodermal progenitor marker and the Flk-1-positive (Flk-1(+)) cells derived from embryonic stem cells (ESCs) have been known to generate hemangioblasts and cardiovascular progenitor cells, which are formed in the early and late stages of differentiation, respectively. In this study, we separated Flk-1(+) and Flk-1(-) cells from spontaneously differentiating embryoid bodies (EBs) of mouse ESCs. We found that cell aggregates derived from late stage Flk-1(+) cells had a relatively small size and a low oxygen consumption rate (OCR) compared with those derived from Flk-1(-) cells. Furthermore, using single-cell comprehensive gene expression analysis, we found that both Flk-1(+) and Flk-1(-) cells could be categorized into subgroups with either low or high glucose metabolic activity. We observed that metabolic suppression occurs in cells expressing an intermediate level of both Nanog and Pou5f1. Taken together, our data suggested that the temporary metabolic suppression is an intrinsic feature of mesodermal differentiation.

Original languageEnglish
Pages (from-to)2560-2567
Number of pages8
JournalMolecular BioSystems
Volume11
Issue number9
DOIs
Publication statusPublished - 2015 Jul 13

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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