Metabolic responses during hemodialysis determined by quantitative 1H NMR spectroscopy

Masako Fujiwara, Itiro Ando, Kazuhisa Takeuchi, Shiro Oguma, Hiroshi Sato, Hiroshi Sekino, Keisuke Sato, Yutaka Imai

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A large proportion of patients with end-stage renal disease have lifelong hemodialysis (HD) treatment. HD rapidly and indiscriminately removes necessary small metabolites together with uremic toxins from plasma into dialysate. To investigate metabolic responses to HD, we determined the levels of metabolites through time-course monitoring of 1H NMR spectroscopy of dialysate during HD. The dialysate sample is stable for analysis because it contains only small metabolites without proteins. It was collected non-invasively from 9 HD patients with chronic glomerular nephropathy, at 6 time points during 4h of HD in 5 sessions. Creatinine, alanine, lactate, pyruvate and valine were simultaneously quantified on a one-dimensional single-pulse spectrum with a single standard compound. The concentration of creatinine exhibited monotonous decay with time, while that of valine decreased slowly and then maintained its levels throughout an HD. Lactate, alanine and pyruvate increased at 2-3h after the initiation of HD. They exhibited remarkable responses to HD with production from the body. The time-course of change in the 4 metabolites of lactate, pyruvate, alanine, and valine had reproducible behavior unique to each patient during the HD. This finding may be applied to distinguish metabolic status in HD patients.

Original languageEnglish
Pages (from-to)159-162
Number of pages4
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume111
DOIs
Publication statusPublished - 2015 Jul 1

Keywords

  • H NMR spectroscopy
  • Hemodialysis
  • Lactate
  • Metabolic response
  • Pyruvate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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