Metabolic assay system for micropatterned contractile myotubes

K. Nagamine, Y. Ido, S. Sekine, T. Miyake, M. Kanzaki, M. Nisizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Contractile C2C12 myotube line patterns embedded in a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. Now, we are investigating contraction-mediated translocation of the GLUT4 glucose transporter protein in myotubes using this system.

Original languageEnglish
Title of host publicationChemical Sensors 9 -and- MEMS/NEMS 9
PublisherElectrochemical Society Inc.
Pages35-39
Number of pages5
Edition8
ISBN (Electronic)9781607681779
ISBN (Print)9781566778275
DOIs
Publication statusPublished - 2010

Publication series

NameECS Transactions
Number8
Volume33
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

ASJC Scopus subject areas

  • Engineering(all)

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