TY - JOUR
T1 - Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector
AU - Gao, Chuanbo
AU - Izquierdo-Barba, Isabel
AU - Nakase, Ikuhiko
AU - Futaki, Shiroh
AU - Ruan, Juanfang
AU - Sakamoto, Kazutami
AU - Sakamoto, Yasuhiro
AU - Kuroda, Kazuyuki
AU - Terasaki, Osamu
AU - Che, Shunai
N1 - Funding Information:
This work was supported by Strategic International Cooperative Project with Sweden (Novel Transdermal Drug Delivery System: Designing Meso-Structured Materials for Controlled and Triggered Release) from Japan Science and Technology Agency (JST) and Swedish Governmental Agency for Innovation Systems (VINNOVA)/Swedish Foundation for Strategic Research (SSF), the National Natural Science Foundation of China (Grant No. 20425102, 20890121 and 20821140537), 973 project (2009CB930403) of China and A3 Foresight Program and NSF. O.T. and Y.S. thank Swedish Research Council (VR) and Japan Science and Technology Agency (JST) for financial support. The authors are grateful to the lab center for basic chemistry, Shanghai Jiao Tong University, for taking the fluorescence spectroscopy.
PY - 2009/6/1
Y1 - 2009/6/1
N2 - A drug delivery system using mesostructured silica as a reservoir has been developed for the storage and controlled release of a drug with a cell-penetrating peptide (CPP) as a vector. We use fluorescein isothiocyanate (FITC) as the drug model and octaarginine (R8) as a vector to endow the drug with cell-penetrating property. The mesostructured silica reservoir system was prepared by using a one-pot liquid-crystal templating method, which is suitable for the encapsulation of intact FITC-R8 conjugates and sustained release of drugs without hampering their properties. The hydrophobic poly(propyl oxide) (PPO) shell of the pore-filling Pluronic F127 and the electrostatic interaction between R8 and siloxide ions on the pore walls act as the diffusion-limiting factors of the FITC-R8 conjugate. A sigmoidal in vitro release of FITC-R8 from mesostructured silica into phosphate buffered saline (PBS, pH 7.4) was observed and the typical release duration was 5 days at 37 °C. Release from the reservoir yielded significant elongation in duration of the FITC signals in DU145 cells by confocal microscopic analysis, compared with a single administration of FITC-R8.
AB - A drug delivery system using mesostructured silica as a reservoir has been developed for the storage and controlled release of a drug with a cell-penetrating peptide (CPP) as a vector. We use fluorescein isothiocyanate (FITC) as the drug model and octaarginine (R8) as a vector to endow the drug with cell-penetrating property. The mesostructured silica reservoir system was prepared by using a one-pot liquid-crystal templating method, which is suitable for the encapsulation of intact FITC-R8 conjugates and sustained release of drugs without hampering their properties. The hydrophobic poly(propyl oxide) (PPO) shell of the pore-filling Pluronic F127 and the electrostatic interaction between R8 and siloxide ions on the pore walls act as the diffusion-limiting factors of the FITC-R8 conjugate. A sigmoidal in vitro release of FITC-R8 from mesostructured silica into phosphate buffered saline (PBS, pH 7.4) was observed and the typical release duration was 5 days at 37 °C. Release from the reservoir yielded significant elongation in duration of the FITC signals in DU145 cells by confocal microscopic analysis, compared with a single administration of FITC-R8.
KW - Cell-penetrating peptide
KW - Drug delivery
KW - Mesostructured silica
KW - Sigmoidal release
KW - Sustained cellular uptake
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U2 - 10.1016/j.micromeso.2009.03.002
DO - 10.1016/j.micromeso.2009.03.002
M3 - Article
AN - SCOPUS:64549087966
VL - 122
SP - 201
EP - 207
JO - Microporous and Mesoporous Materials
JF - Microporous and Mesoporous Materials
SN - 1387-1811
IS - 1-3
ER -